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A comprehensive analysis of Candida albicans phosphoproteome reveals dynamic changes in phosphoprotein abundance during hyphal morphogenesis

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Title A comprehensive analysis of Candida albicans phosphoproteome reveals dynamic changes in phosphoprotein abundance during hyphal morphogenesis
 
Creator Ghorai, Priyanka
Irfan, Mohammad
Narula, Alka
Datta, Asis
 
Subject Candida albicans
Hyphal development
Phosphoproteome
TiO2
LC–MS/MS
Protein kinase
 
Description Accepted date: 2 August 2018
The morphological plasticity of Candida albicans is a virulence determinant as the hyphal form has significant roles in the infection process. Recently, phosphoregulation of proteins through phosphorylation and dephosphorylation events has gained importance in studying the regulation of pathogenicity at the molecular level. To understand the importance of phosphorylation in hyphal morphogenesis, global analysis of the phosphoproteome was performed after hyphal induction with elevated temperature, serum, and N-acetyl-glucosamine (GlcNAc) treatments. The study identified 60, 20, and 53 phosphoproteins unique to elevated temperature-, serum-, and GlcNAc-treated conditions, respectively. Distribution of unique phosphorylation sites sorted by the modified amino acids revealed that predominant phosphorylation occurs in serine, followed by threonine and tyrosine residues in all the datasets. However, the frequency distribution of phosphorylation sites in the proteins varied with treatment conditions. Further, interaction network-based functional annotation of protein kinases of C. albicans as well as identified phosphoproteins was performed, which demonstrated the interaction of kinases with phosphoproteins during filamentous growth. Altogether, the present findings will serve as a base for further functional studies in the aspects of protein kinase-target protein interaction in effectuating phosphorylation of target proteins, and delineating the downstream signaling networks linked to virulence characteristics of C. albicans.
This work is financially supported by grants from
Council of Scientific and Industrial Research, Department of
Biotechnology and the Core Grant of National Institute of Plant
Genome Research, New Delhi, India. P.G. and M.I. acknowledge
Council of Scientific and Industrial Research, India, for the Senior
Research Fellowship and Senior Research Associateship, respectively.
 
Date 2018-08-24T06:41:49Z
2018-08-24T06:41:49Z
2018
 
Type Article
 
Identifier Applied Microbiology and Biotechnology, 102(22): 9731-9743
1432-0614
http://223.31.159.10:8080/jspui/handle/123456789/882
https://link.springer.com/article/10.1007%2Fs00253-018-9303-z
https://doi.org/10.1007/s00253-018-9303-z
 
Language en_US
 
Format application/pdf
 
Publisher Springer Nature