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Expression dynamics of miRNAs and their targets in seed germination conditions reveals miRNA-ta-siRNA crosstalk as regulator of seed germination

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Title Expression dynamics of miRNAs and their targets in seed germination conditions reveals miRNA-ta-siRNA crosstalk as regulator of seed germination
 
Creator Das, Shabari Sarkar
Yadav, Sandeep
Singh, Archita
Gautam, Vibhav
Sarkar, Ananda K.
Nandi, Asis K.
Karmakar, Prakash
Majee, Manoj
Sanan-Mishra, Neeti
 
Subject Plant physiology
Plant biotechnology
miRNAs
seed germination
miRNA-ta-siRNA crosstalk
 
Description Accepted date: 5 December 2017
Seed germination paves the way for the dormant embryo to establish itself as a new plant marking the first critical step in postembryonic plant growth and development. Germination starts with the uptake of water (imbibition), followed by induction of transcription, translation, energy metabolism, and cell division processes. Although small RNAs have been implicated in many developmental processes, their role during seed germination stages and conditions remained elusive. Here we show that seed germination conditions, like imbibition and temperature, dynamically regulate the expression of many developmentally important miRNAs and their targets. We have identified 58 miRNAs belonging to 30 different families at different seed germination conditions. Amongst these, 15 miRNAs and their targets were significantly differentially expressed in Arabidopsis seeds in dry and 12 h, 24 h and 48 h of imbibition. Interestingly, differential expression of miR390, which targets trans-acting siRNA locus (TAS3) derived transcripts, resulted in alteration of tasiR-ARF mediated regulation of expression of target AUXIN RESPONSE FACTORs (ARF2/3/4). Our results suggest that the dynamic expression of several miRNAs, their targets, and a crosstalk between miRNA and ta-siRNA pathways contribute to the regulation of seed germination in Arabidopsis thaliana.
SSD thanks Department of Science and Technology (DST, India) for the Woman Scientist-A (WOS-A) fellowship
(grant no. WOS-A/LS-1276/2014) and National Institute of Plant Genome Research (NIPGR) for short-term
fellowship. SSD also thanks International Centre for Genetic Engineering and Biotechnology (ICGEB) for
instrument and basic lab facilities during WOS-A fellowship. AKS and MM acknowledge Central Instrument
Facility (CIF) and financial support from NIPGR. Fellowship to SY was provided by University Grant
Commission (UGC, India) and fellowship to AS and VG was provided by Council of Scientifc and Industrial
Research (CSIR, India). We also thank M. Karthikeyan for help in preliminary stage of the work.
 
Date 2018-01-23T07:32:09Z
2018-01-23T07:32:09Z
2018
 
Type Article
 
Identifier Scientific Reports, 8(1): 1233
2045-2322
http://223.31.159.10:8080/jspui/handle/123456789/829
https://www.nature.com/articles/s41598-017-18823-8?WT.feed_name=subjects_plant-biotechnology
10.1038/s41598-017-18823-8
 
Language en_US
 
Format application/pdf
 
Publisher Nature Publishing Group