Record Details

A protein phosphatase 2C, AP2C1 interacts with and negatively regulates the function of CIPK9 under potassium deficient conditions in Arabidopsis

NIPGR Digital Knowledge Repository (NDKR)

View Archive Info
 
 
Field Value
 
Title A protein phosphatase 2C, AP2C1 interacts with and negatively regulates the function of CIPK9 under potassium deficient conditions in Arabidopsis
 
Creator Singh, Amarjeet
Yadav, Akhilesh K.
Kaur, Kanwaljeet
Sanyal, Sibaji K.
Jha, Saroj K.
Fernandes, Joel L.
Sharma, Pankhuri
Tokas, Indu
Pandey, Amita
Luan, Sheng
Pandey, Girdhar K.
 
Subject Arabidopsis
CBL-interacting protein kinase
Calcium signaling
Dephosphorylation
Phosphorylation
Potassium deficiency
Protein phosphatase 2C
Regulation
 
Description Accepted date: 8 May 2018
Potassium (K+) is a major macronutrient required for plant growth. In response to low- K+ condition, an adaptive mechanism entails activation of the Ca2+ signaling network consisting of calcineurin B-like proteins (CBLs) and their interacting kinases (CIPKs) in plants. The CBL-interacting protein kinase 9 (CIPK9) is previously implicated in low-K+ responses in Arabidopsis thaliana. Here, we report a protein phosphatase 2C (PP2C), AP2C1, as an interactor of CIPK9. Fluorescence resonance energy transfer (FRET), bimolecular fluorescence complementation (BiFC) and co-localization analyses revealed that CIPK9 and AP2C1 interact in the cytoplasm. AP2C1 dephosphorylates the auto-phosphorylated form of CIPK9 in vitro, presenting a regulatory mechanism for CIPK9 function. Furthermore, genetic and molecular analysis revealed that ap2c1 null mutants (ap2c1-1 and ap2c1-2) are tolerant to low-K+ conditions, retained higher K+ content and showed higher expression of K+ deficiency related genes contrary to cipk9 mutants (cipk9-1 and cipk9-2). In contrast, transgenic plants overexpressing AP2C1 were sensitive to low-K+ conditions. Thus, this study shows that AP2C1 and CIPK9 interact to regulate K+-deficiency responses in Arabidopsis. CIPK9 functions as positive regulator whereas, AP2C1 acts as a negative regulator of Arabidopsis root growth and seedling development under low-K+ conditions.
We are thankful to Prof. Jörg Kudla (Universität Münster, Germany) for providing the
pGPTVII.GFP.Kan, pSPYCE-35SGW and pSPYNE-35SGW vectors; Prof. Michael Goodin
(University of Kentucky, USA) for pSITE 4CA vectors; and Dr. Irute Meskiene (Max F. Perutz
Laboratories, University of Vienna, Austria) for providing ap2c1-1 (SALK_065126). Arabidopsis
Biological Resource Center, Ohio is acknowledged for providing the ap2c1-2 T-DNA insertion
allele (SALK_104445). We also express our thanks to Dr. Kailash C. Pandey (National Institute for
Research in Environmental Health, India) for critical reading and comments on this manuscript. We
are thankful to Department of Biotechnology (DBT) and University Grant Commission (UGC;
UGC-SAP grant), India for research support (in GKP’s lab). AS, AKY, IT acknowledge Council of
Scientific and Industrial Research (CSIR), India for financial support through research fellowships.
 
Date 2018-05-16T09:41:07Z
2018-05-16T09:41:07Z
2018
 
Type Article
 
Identifier Journal of Experimental Botany, 69(16): 4003-4015
1460-2431
http://223.31.159.10:8080/jspui/handle/123456789/857
https://academic.oup.com/jxb/advance-article/doi/10.1093/jxb/ery182/4996122
https://doi.org/10.1093/jxb/ery182
 
Language en_US
 
Format application/pdf
 
Publisher Oxford University Press