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Using different forms of nitrogen to study hypersensitive response elicited by avirulent Pseudomonas syringae

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Title Using different forms of nitrogen to study hypersensitive response elicited by avirulent Pseudomonas syringae
 
Creator Singh, Namrata
Singh, Pooja
Pathak, Pradeep Kumar
Gupta, Kapuganti Jagadis
 
Subject Nitrate
Ammonium
Nitric oxide
Reactive oxygen species
Hypersensitive response
Pseudomonas syringae
 
Description Accepted date: 09 October 2019
Nitrate, ammonium, or a combination of both is the form of N available for nitrogen assimilation from soil by the plants. Nitrogen is an important and integral part of amino acids, nucleotides, and defense molecules. Hence it is very important to study the role of nitrate and ammonium nutrition in plant defense via hypersensitive response (HR). Shifting plants from ammonium nitrate Hoagland solution to nitrate Hoagland nutrition slightly enhances root length and leaf area. HR phenotype is different in nitrate and ammonium grown plants when challenged with avirulent Pseudomonas syringae DC3000 avrRpm1. HR is also associated with increased production of reactive oxygen species (ROS) and nitric oxide (NO). Hence to understand HR development it is essential to measure HR lesions, cell death, ROS, NO, and bacterial growth. Here we provide a stepwise protocol of various parameters to study HR in Arabidopsis in response to nitrate and ammonium nutrition.
NS is a recipient of JRF and SRF from CSIR, India. PS and PKP are recipients of UGC JRF and SRF. This work is supported by SERB Early Carrier Research Award to KJG by SERB, Govt. of India.
 
Date 2019-10-15T07:49:09Z
2019-10-15T07:49:09Z
2020
 
Type Book chapter
 
Identifier Methods in Molecular Biology, 2057: 79-92
978-1-4939-9790-9
http://223.31.159.10:8080/jspui/handle/123456789/1002
https://link.springer.com/protocol/10.1007%2F978-1-4939-9790-9_8
https://doi.org/10.1007/978-1-4939-9790-9_8
 
Language en_US
 
Format application/pdf
 
Publisher Springer Nature Publishing AG