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Novel and conserved functions of S-nitrosoglutathione reductase in tomato

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Title Novel and conserved functions of S-nitrosoglutathione reductase in tomato
 
Creator Hussain, Adil
Yun, Byung-Wook
Kim, Ji Hyun
Gupta, Kapuganti Jagadis
Hyung, Nam-In
Loake, Gary J.
 
Subject Climacteric fruit
fruit development
GSNOR
MicroTom
nitric oxide
NO
S-nitrosation
S-nitrosylation
tomato
tomato fruit
 
Description Accepted date: 29 April 2019
Nitric oxide (NO) is emerging as a key signalling molecule in plants. The chief mechanism for the transfer of NO
bioactivity is thought to be S-nitrosylation, the addition of an NO moiety to a protein cysteine thiol to form an
S-nitrosothiol (SNO). The enzyme S-nitrosoglutathione reductase (GSNOR) indirectly controls the total levels of cellular S-nitrosylation, by depleting S-nitrosoglutathione (GSNO), the major cellular NO donor. Here we show that depletion of GSNOR function impacts tomato (Solanum lycopersicum. L) fruit development. Thus, reduction of GSNOR
expression through RNAi modulated both fruit formation and yield, establishing a novel function for GSNOR. Further,
depletion of S. lycopersicum GSNOR (SlGSNOR) additionally impacted a number of other developmental processes,
including seed development, which also has not been previously linked with GSNOR activity. In contrast to Arabidopsis,
depletion of GSNOR function did not influence root development. Further, reduction of GSNOR transcript abundance
compromised plant immunity. Surprisingly, this was in contrast to previous data in Arabidopsis that reported that reducing Arabidopsis thaliana GSNOR (AtGSNOR) expression by antisense technology increased disease resistance.
We also show that increased SlGSNOR expression enhanced pathogen protection, uncovering a potential strategy to
enhance disease resistance in crop plants. Collectively, our findings reveal, at the genetic level, that some but not all
GSNOR activities are conserved outside the Arabidopsis reference system. Thus, manipulating the extent of GSNOR
expression may control important agricultural traits in tomato and possibly other crop plants.
AH was supported by Higher Education Commission (HEC) Pakistan
for PhD Studentship. Research in the laboratory of GJL has been supported by BBSRC grant BB/DO11809/1.
 
Date 2019-09-25T07:50:16Z
2019-09-25T07:50:16Z
2019
 
Type Article
 
Identifier Journal of Experimental Botany 70(18): 4877–4886
1460-2431
http://223.31.159.10:8080/jspui/handle/123456789/988
https://academic.oup.com/jxb/article/70/18/4877/5489421
https://doi.org/10.1093/jxb/erz234
 
Language en_US
 
Format application/pdf
 
Publisher Oxford University Press