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GC-MS-based analysis of methanol: chloroform-extracted fatty acids from plant tissues

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Title GC-MS-based analysis of methanol: chloroform-extracted fatty acids from plant tissues
 
Creator Patel, Manish Kumar
Das, Shubhashis
Thakur, Jitendra K.
 
Subject Plant tissue
Nutraceutical
Lipids
GC-MS
Fatty acids
Biodiesel
 
Description Accepted date: 20 Sept 2018
Fatty acids (FAs) are carboxylic acids with long aliphatic chains that may be straight, branched and saturated or unsaturated. Most of the naturally occurring plant FAs contains an even number of carbon (C4-C24). FAs are used in food and pharmacological industries due to their nutritional importance. In addition, FAs are considered as a promising alternative for the production of biodiesel from terrestrial plant biomass. To establish commercial applications, more reliable analytical methods are needed for the identification, quantification, and composition determination of FAs. Here, we describe a relatively rapid and sensitive method for the extraction, identification, and quantification of FAs from a small quantity of plant tissue. The method includes steps of lipid extraction, conversion of lipid to fatty acid methyl esters (FAMEs) by transmethylation, identification and quantification of FAMEs using gas chromatography-mass spectrometry (GC-MS). In this protocol, an internal standard is added prior to GC-MS analysis. The amount of each FA is calculated from its peak area relative to the peak area of the internal standard.
MKP acknowledges the National Post-doctoral Fellowship (PDF/2016/001971) from SERB-Department of Science & Technology (DST), Govt. of India for supporting this work. Core grant from National Institute of Plant Genome Research (NIPGR) is also acknowledged. SD acknowledges junior research fellowship from Department of Biotechnology (DBT), Govt. of India. We acknowledge DeLCON for research literature made available to us. This protocol has been derived from Bligh and Dyer (1959) and Carreau and Dubacq (1978) with minor modifications made by Mishra et al. (2015), Pandey et al. (2015) and Patel et al. (2016) for reproducible results.
 
Date 2019-02-26T07:05:21Z
2019-02-26T07:05:21Z
2018
 
Type Article
 
Identifier Bio-Protocol, 8(18): e3014
2331-8325
http://223.31.159.10:8080/jspui/handle/123456789/922
https://bio-protocol.org/e3014
10.21769/BioProtoc.3014
 
Language en_US
 
Publisher Bio-protocol LLC.