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Petal abscission in roses is associated with the activation of a truncated version of the animal PDCD4 homologue, RbPCD1

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Title Petal abscission in roses is associated with the activation of a truncated version of the animal PDCD4 homologue, RbPCD1
 
Creator Singh, Priya
Singh, Amar Pal
Tripathi, Siddharth Kaushal
Kumar, Vinod
Sane, Aniruddha P.
 
Subject Ethylene
PDCD4
MA3 domain
Repression
Senescence
Inflorescence
Heat shock
roses
Petal abscission
 
Description Accepted date: 27 August 2019
Abscission is a developmental process that leads to shedding of organs not needed by the plant. Apart from wallhydrolysis, the cells of the abscission zone (AZ) are also believed to undergo programmed cell death (PCD). Weshow that ethylene-induced petal abscission inRosa bourbonianais accompanied with the activation ofRbPCD1(PROGRAMMED CELL DEATH LIKE 1) encoding a protein of 78 amino acids. Its expression increases duringnatural and ethylene-induced petal abscission. Its transcription in most tissues is up-regulated by ethylene.RbPCD1 shows similarity to the N-terminal domain of animal PDCD4 (PROGRAMMED CELL DEATH PROTEIN 4)proteins that are activated during apoptosis and function as transcriptional and translational repressors. RbPCD1resides in the nucleus and cytoplasm and acts as a transcriptional repressor. Constitutive expression ofRbPCD1intransgenic Arabidopsis is seedling lethal. Heat-induced expression ofRbPCD1under the soybean heat-shockpromoter affects leaf function, inflorescence development, silique formation, seed yield and reduces survival.Nuclear localization of RbPCD1 is necessary for manifestation of its effects. RbPCD1 may be necessary to mediatesome of the ethylene-induced changes during abscission and senescence in specific tissues.
We are grateful to Prof. Shucai Wang (Northeast Normal Univ,Changchun, China) for effector and reporter vectors for protoplasttransfection study and to Dr Ute Hoecker (Univ of Cologne, Germany)for the gift of the pRTL.2 vector. We thank Dr Sadaf Khan (a former postdoc in our lab) and Dr PA Shirke (Dept of Plant Physiology, CSIR-NBRI)forhelp with the PAM-Imaging. We are grateful to Mr Ram Awadh fortaking care of the rose plants. PS, APS, SKT and VK were supported by the Council of Scientific and Industrial Research, New Delhi with SeniorResearch Fellowships. The work was supported by a Department ofBiotechnology, Govt. of India funded project to APS.
 
Date 2019-09-17T05:15:59Z
2019-09-17T05:15:59Z
2019
 
Type Article
 
Identifier Plant Science, 288: 110242
0168-9452
http://223.31.159.10:8080/jspui/handle/123456789/983
https://www.sciencedirect.com/science/article/pii/S0168945219310854
https://doi.org/10.1016/j.plantsci.2019.110242
 
Language en_US
 
Format application/pdf
 
Publisher Elsevier B.V.