Record Details

Targeted silencing of genes in polyploids: lessons learned from Brassica juncea-glucosinolate system

NIPGR Digital Knowledge Repository (NDKR)

View Archive Info
 
 
Field Value
 
Title Targeted silencing of genes in polyploids: lessons learned from Brassica juncea-glucosinolate system
 
Creator Augustine, Rehna
Bisht, Naveen C.
 
Subject Brassica
Glucosinolates
BjuMYB28
Gene-silencing
 
Description Accepted date: 2nd October 2018
Key message Intron-spliced hairpin RNAi construct targeting the exonic region of BjuMYB28 driven by the native
promoter is the best suited strategy for developing viable low glucosinolate lines in polyploid Brassica juncea.
Abstract Targeted silencing of specific homolog(s) of a multigene family in polyploids through RNA interference (RNAi)
is a challenging effort. Indian oilseed mustard (Brassica juncea), a natural allotetraploid, is expected to have 4–6 copies of
every Arabidopsis gene ortholog. In the current study, we have attempted to establish the best gene silencing system suitable
for BjuMYB28, a transcription factor gene, with the objective of developing low seed glucosinolate lines in B. juncea.
After comparing multiple combinations of BjuMYB28 gene homologs, promoters, target regions (exon and 3′ UTR) and
silencing strategies (RNAi and antisense), we suggest that the intron-spliced hairpin RNAi construct targeting the specific
exonic region of the BjuMYB28 gene under the control of native promoter, whose peak activity synchronises with the highest
glucosinolate accumulation phase of the plant, is the best suited strategy for developing viable low glucosinolate lines
in polyploid B. juncea.
Research was supported by project grants (BT/
PR10268/27/81/2007 and BT/PR2171/AGR/36/687/2011) from DBT
(India) to NCB. RA was supported from JRF of CSIR (India) and Short
Term Research Fellowship of NIPGR (New Delhi).
 
Date 2018-10-22T11:14:29Z
2018-10-22T11:14:29Z
2019
 
Type Article
 
Identifier Plant Cell Reports, 38(1): 51-57
1432-203X
http://223.31.159.10:8080/jspui/handle/123456789/892
https://link.springer.com/article/10.1007%2Fs00299-018-2348-8
https://doi.org/10.1007/s00299-018-2348-8
 
Language en_US
 
Format application/pdf
 
Publisher Springer Nature