Record Details

Nitrate nutrition influences multiple factors in order to increase energy efficiency under hypoxia in Arabidopsis

NIPGR Digital Knowledge Repository (NDKR)

View Archive Info
 
 
Field Value
 
Title Nitrate nutrition influences multiple factors in order to increase energy efficiency under hypoxia in Arabidopsis
 
Creator Wany, Aakanksha
Gupta, Alok Kumar
Kumari, Aprajita
Mishra, Sonal
Singh, Namrata
Pandey, Sonika
Vanvari, Rhythm
Igamberdiev, Abir U.
Fernie, Alisdair R.
Gupta, Kapuganti Jagadis
 
Subject Arabidopsis thaliana
nitrate
ammonium
alternative oxidase
nitric oxide
fermentation
phytoglobin
 
Description Accepted date: 10 October 2018
BACKGROUND AND AIMS:
Nitrogen (N) levels vary between ecosystems, while the form of available N has a substantial impact on growth, development and perception of stress. Plants have the capacity to assimilate N in the form of either nitrate (NO3-) or ammonium (NH4+). Recent studies revealed that NO3- nutrition increases nitric oxide (NO) levels under hypoxia. When oxygen availability changes, plants need to generate energy to protect themselves against hypoxia-induced damage. As the effects of NO3- or NH4+ nutrition on energy production remain unresolved, this study was conducted to investigate the role of N source on group VII transcription factors, fermentative genes, energy metabolism and respiration under normoxic and hypoxic conditions.

METHODS :
We used Arabidopsis plants grown on Hoagland medium with either NO3- or NH4+ as a source of N and exposed to 0.8 % oxygen environment. In both roots and seedlings, we investigated the phytoglobin-nitric oxide cycle and the pathways of fermentation and respiration; furthermore, NO levels were tested using a combination of techniques including diaminofluorescein fluorescence, the gas phase Griess reagent assay, respiration by using an oxygen sensor and gene expression analysis by real-time quantitative reverse transcription-PCR methods.

KEY RESULTS :
Under NO3- nutrition, hypoxic stress leads to increases in nitrate reductase activity, NO production, class 1 phytoglobin transcript abundance and metphytoglobin reductase activity. In contrast, none of these processes responded to hypoxia under NH4+ nutrition. Under NO3- nutrition, a decreased total respiratory rate and increased alternative oxidase capacity and expression were observed during hypoxia. Data correlated with decreased reactive oxygen species and lipid peroxidation levels. Moreover, increased fermentation and NAD+ recycling as well as increased ATP production concomitant with the increased expression of transcription factor genes HRE1, HRE2, RAP2.2 and RAP2.12 were observed during hypoxia under NO3- nutrition.

CONCLUSIONS:
The results of this study collectively indicate that nitrate nutrition influences multiple factors in order to increase energy efficiency under hypoxia.
This work was supported by a Ramalingaswami Fellowship
and an IYBA award funded to K.J.G. by the Department of
Biotechnology, Government of India, SERB-NPDF and NIPGR
STRF to A.W., SERB-NPDF to S.P., CSIR JRF fellowship to
N.S. and IYBA-JRF to A.K. The authors have no conflicts of
interest to declare.
 
Date 2018-12-13T06:44:57Z
2018-12-13T06:44:57Z
2019
 
Type Article
 
Identifier Annals of Botany, 123(4): 691-705
1095-8290
http://223.31.159.10:8080/jspui/handle/123456789/903
https://academic.oup.com/aob/advance-article/doi/10.1093/aob/mcy202/5237733
https://doi.org/10.1093/aob/mcy202
 
Language en_US
 
Format application/pdf
 
Publisher Oxford University Press