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IBR5 regulates leaf serrations development via modulation of the expression of PIN1

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Title IBR5 regulates leaf serrations development via modulation of the expression of PIN1
 
Creator Kong, Xiuzhen
Huang, Guoqiang
Xiong, Yali
Zhao, Chunyan
Wang, Jun
Song, Xiaoyun
Giri, Jitender
Zuo, Kaijing
 
Subject Arabidopsis
leaf serrations
IBR5
auxin maxima
PIN1
 
Description Accepted date: 6 September 2019
Biodiversity in plant shape is mainly attributable to the diversity of leaf shape, which
is largely determined by the transient morphogenetic activity of the leaf margin that creates leaf
serrations. However, the precise mechanism underlying the establishment of this morphogenetic
capacity remains poorly understood. We report here that INDOLE-3-BUTYRIC ACID RESPONSE 5
(IBR5), a dual-specificity phosphatase, is a key component of leaf-serration regulatory machinery.
Loss-of-function mutants of IBR5 exhibited pronounced serrations due to increased cell area. IBR5
was localized in the nucleus of leaf epidermis and petiole cells. Introducing a C129S mutation
within the highly conserved VxVHCx2GxSRSx5AYLM motif of IBR5 rendered it unable to rescue the
leaf-serration defects of the ibr5-3 mutant. In addition, auxin reporters revealed that the distribution of
auxin maxima was expanded ectopically in ibr5-3. Furthermore, we found that the distribution of PIN1
on the plasma membrane of the epidermal and cells around the leaf vein was compromised in ibr5-3.
We concluded that IBR5 is essential for the establishment of PIN-FORMED 1 (PIN1)-directed auxin
maxima at the tips of leaf serration, which is vital for the elaborated regulation during its formation.
We thank the NASC for kindly providing the plant materials described in this article.
We thank Hongwei Xue for providing DR5-GUS, DR5-GFP, and PIN1-GFP transgenic seeds.
 
Date 2019-09-16T05:37:13Z
2019-09-16T05:37:13Z
2019
 
Type Article
 
Identifier International Journal of Molecular Sciences, 20(18): 4429
1422-0067
http://223.31.159.10:8080/jspui/handle/123456789/982
https://www.mdpi.com/1422-0067/20/18/4429
https://doi.org/10.3390/ijms20184429
 
Language en_US
 
Format application/pdf
 
Publisher MDPI AG