The nodule-specific PLAT-domain protein NPD1 is required for nitrogen-fixing symbiosis
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Title |
The nodule-specific PLAT-domain protein NPD1 is required for nitrogen-fixing symbiosis
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Creator |
Pislariu, Catalina I.
Sinharoy, Senjuti Torres-Jerez, Ivone Nakashima, Jin Blancaflor, Elison B. Udvardi, Michael K |
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Subject |
PLAT-domain protein
nitrogen-fixing symbiosis nodule-specific |
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Description |
Accepted date: April 22, 2019
Symbiotic nitrogen fixation by rhizobia in legume root nodules is a key source of nitrogen for sustainable agriculture. Genetic approaches have revealed important roles for only a few of the thousands of plant genes expressed during nodule development and symbiotic nitrogen fixation. Previously, we isolated over one hundred nodulation and nitrogen fixation mutants from a population of Tnt1-insertion mutants of Medigaco truncatula (Pislariu et al., 2011). Using Tnt1 as a tag to identify genetic lesions in these mutants, we discovered that insertions in a nodule-specific PLAT (Polycystin-1, Lipoxygenase, Alpha-Toxin) domain-encoding gene, MtNPD1, resulted in development of ineffective nodules. Early stages of nodule development and colonization by the nitrogen fixing bacterium Sinorhizobium meliloti appeared to be normal in the npd1 mutant. However, npd1 nodules ceased to grow after a few days, resulting in abnormally small, ineffective nodules. Rhizobia that colonized developing npd1 nodules did not differentiate completely into nitrogen-fixing bacteroids and quickly degraded. MtNPD1 expression was low in roots but increased significantly in developing nodules four days post-inoculation (DPI), and expression accompanied invading rhizobia in the nodule infection zone and into the distal nitrogen fixation zone. A functional MtNPD1:GFP fusion protein localized in the space surrounding symbiosomes in infected cells. When ectopically expressed in tobacco (Nicotiana tabacum) leaves, MtNPD1 co-localized with vacuoles and the endoplasmic reticulum. MtNPD1 belongs to a cluster of 5 nodule-specific single PLAT domain-encoding genes, with apparent non-redundant functions. This work was funded by the National Science Foundation Plant Genome Research Program (grants DBI-0703285 and DBI-1127155), the National Science Foundation Major Research Instrumentation (MRI) Program (grant DBI-0722635), and by the Noble Research Institute. |
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Date |
2019-05-17T05:26:19Z
2019-05-17T05:26:19Z 2019 |
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Type |
Article
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Identifier |
Plant Physiology, 180(3): 1480-1497
1532-2548 http://223.31.159.10:8080/jspui/handle/123456789/950 http://www.plantphysiol.org/content/early/2019/05/06/pp.18.01613.long https://doi.org/10.1104/pp.18.01613 |
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Language |
en_US
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Format |
application/pdf
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Publisher |
American Society of Plant Biologists
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