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A novel glycerophosphodiester phosphodiesterase improves phosphate deficiency tolerance in rice

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Title A novel glycerophosphodiester phosphodiesterase improves phosphate deficiency tolerance in rice
 
Creator Mehra, Poonam
Pandey, Bipin K.
Verma, Lokesh
Giri, Jitender
 
Subject Pi deficiency
phosphorus‐use efficiency
transgenics
Oryza sativa
lipid remodeling
root
 
Description Accepted date: 7 October 2018
Soil phosphate (Pi) deficiency is major constraint for rice cultivation world‐wide. Cellular membranes account for one‐third of cellular P (Phosphorus) in the form of phospholipids. Therefore, remobilization of Pi from membrane phospholipids under Pi deficiency can be an important strategy to improve PUE (Phosphorus Use Efficiency). GDPDs (Glycerophosphodiester phosphodiesterases) hydrolyse intermediate product of phospholipid catabolism, glycerophosphodiesters to glycerol‐3‐phosphate (G3P); a precursor for P and non P‐lipid biosynthesis. Here, we show that OsGDPD2 is a Pi deficiency responsive gene which is transcriptionally regulated by OsPHR2. In silico analysis of active site residues and enzymatic assays confirmed phosphodiesterase activity of OsGDPD2. All overexpression lines showed higher GDPD activity, Pi content, root growth and biomass accumulation as compared to wild‐type. Conversely, silencing of OsGDPD2 led to decreased GDPD activity and Pi content. Notably, most of the P‐containing metabolites and fatty acids were elevated in transgenic lines. Further, quantitative analysis of polar lipids revealed higher accumulation of several classes of phospholipids and galactolipids in overexpression lines indicating a potential role of OsGDPD2 in de novo glycerolipid biosynthesis. Thus, present study provides insights into novel physiological roles of OsGDPD2 in low Pi acclimation in rice.
P.M., B.K.P, L.V acknowledge financial assistance from NIPGR, DBT, and CSIR,
respectively. JG acknowledges a grant from DBT-IYBA project. Authors acknowledge
Kansas Lipidomics Research Center for performing lipid profiling.
 
Date 2018-10-22T10:35:50Z
2018-10-22T10:35:50Z
2019
 
Type Article
 
Identifier Plant, Cell & Environment, 42(4): 1167-1179
1365-3040
http://223.31.159.10:8080/jspui/handle/123456789/891
https://onlinelibrary.wiley.com/doi/abs/10.1111/pce.13459
https://doi.org/10.1111/pce.13459
 
Language en_US
 
Format application/pdf
 
Publisher John Wiley & Sons