Record Details

A tripartite interaction among the basidiomycete Rhodotorula mucilaginosa, N2-fixing endobacteria, and rice improves plant nitrogen nutrition

NIPGR Digital Knowledge Repository (NDKR)

View Archive Info
 
 
Field Value
 
Title A tripartite interaction among the basidiomycete Rhodotorula mucilaginosa, N2-fixing endobacteria, and rice improves plant nitrogen nutrition
 
Creator Paul, Karnelia
Saha, Chinmay
Nag, Mayurakshi
Mandal, Drishti
Naiya, Haraprasad
Sen, Diya
Mitra, Souvik
Kumar, Mohit
Bose, Dipayan
Mukherjee, Gairik
Naskar, Nabanita
Lahiri, Susanta
Ghosh, Upal Das
Tripathi, Sudipta
Sarkar, Mousumi Poddar
Banerjee, Manidipa
Kleinert, Aleysia
Valentine, Alexander J.
Tripathy, Sucheta
Sinharoy, Senjuti
Seal, Anindita
 
Subject endophyte
nitrogen metabolism
Oryza sativa
plant growth promotion
Rhodotorula mucilaginosa
 
Description Accepted date: November 19, 2019
Nitrogen (N) limits crop yield and improvement of N nutrition remains a key goal for crop research; one approach to improve N nutrition is identifying plant-interacting N2-fixing microbes. Rhodotorula mucilaginosa JGTA-S1 is a basidiomycetous yeast endophyte of narrowleaf cattail (Typha angustifolia). JGTA-S1 could not convert nitrate or nitrite to ammonium, but harbors diazotrophic (N2-fixing) endobacteria (eg. Pseudomonas stutzeri) that allows JGTA-S1 to fix N2 and grow in a N-free environment; moreover, P. stutzeri dinitrogen reductase (nifH) was transcribed in JGTA-S1 even under adequate N. Endobacteria-deficient JGTA-S1 had reduced fitness, which was restored by reintroducing P. stutzeri. JGTA-S1 colonizes rice (Oryza sativa) significantly improving its growth, N content, and relative N-use efficiency. Endofungal P. stutzeri plays a significant role in increasing the biomass and ammonium content of rice treated with JGTA-S1; also, JGTA-S1 has better N2 fixing ability than free-living P. stutzeri and provides fixed N to the plant. Genes involved in N metabolism, N transporters, and NODULE INCEPTION (NIN)-like transcription factors were upregulated in rice roots within 24 h of JGTA-S1 treatment. In association with rice, JGTA-S1 has a filamentous phase and P. stutzeri only penetrated filamentous JGTA-S1. Together, these results demonstrate an interkingdom interaction that improves rice N nutrition.
We acknowledge the Department of Biotechnology project number BT/PR15410/BCE/08/861/2011 for funding the work. Karnelia Paul’s fellowship was funded
by UGC-UPE grant of CU. We acknowledge Madhumanti Das for her help in the
characterization of Rhodotorula. We thank Prof. Maitrayee Dasgupta, Department of
Biochemistry C.U. for the LIVE DEAD bacterial stain. We also acknowledge DBT IPLS, the
University of Calcutta for the Confocal Microscopy facility and Arijit Pal and Souvik Roy for
their technical assistance in microscopy.
 
Date 2019-11-27T09:44:18Z
2019-11-27T09:44:18Z
2020
 
Type Article
 
Identifier Plant Cell, 32: 486–507
1531-298X
http://223.31.159.10:8080/jspui/handle/123456789/1016
http://www.plantcell.org/content/early/2019/11/22/tpc.19.00385.long
https://doi.org/10.1105/tpc.19.00385
 
Language en_US
 
Format application/pdf
 
Publisher American Society of Plant Biologists