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Dissecting the chloroplast proteome of chickpea (Cicer arietinum L.) provides new insights into classical and non-classical functions

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Title Dissecting the chloroplast proteome of chickpea (Cicer arietinum L.) provides new insights into classical and non-classical functions
 
Creator Lande, Nilesh Vikram
Subba, Pratigya
Barua, Pragya
Gayen, Dipak
Keshava Prasad, T.S.
Chakraborty, Subhra
Chakraborty, Niranjan
 
Subject Chloroplast proteins
Grain legume
Global profiling
LC-MS/MS analysis
Protein repertoire
Transit peptides
 
Description Accepted date: 3 June 2017
Chloroplast, the energy organelle unique to plant cells, is a dynamic entity which integrates an array of metabolic pathways and serves as first level for energy conversion for the entire ecological hierarchy. Increasing amount of sequence data and evolution of mass spectrometric approaches has opened up new avenues for opportune exploration of the global proteome of this organelle. In our study, we aimed at generation of a comprehensive catalogue of chloroplast proteins in a grain legume, chickpea and provided a reference proteome map. To accurately assign the identified proteins, purity of chloroplast-enriched fraction was stringently monitored by multiple chemical and immunological indexes, besides pigment and enzyme analyses. The proteome analysis led to the identification of 2451 proteins, including 27 isoforms, which include predicted and novel chloroplast constituents. The identified proteins were validated through their sequence analysis. Extensive sequence based localization prediction revealed more than 50% proteins to be chloroplast resident by at least two different algorithms. Chromosomal distribution of identified proteins across nuclear and chloroplast genome unveiled the presence of 55 chloroplast encoded gene. In depth comparison of our dataset with the non-redundant set of chloroplast proteins identified so far across other species revealed novel as well as overlapping candidates.
This work was supported by the Department of Biotechnology (DBT) (BT/AGR/CG-Phase II/01/2014), Govt. of India. The authors thank Council of Scientific and Industrial Research (CSIR) and DBT for providing research fellowship to NVL and PB and DG, respectively.
 
Date 2017-06-20T06:03:15Z
2017-06-20T06:03:15Z
2017
 
Type Article
 
Identifier Journal of Proteomics, 165: 11-20
1874-3919
http://59.163.192.83:8080/jspui/handle/123456789/759
http://www.sciencedirect.com/science/article/pii/S1874391917302087
https://doi.org/10.1016/j.jprot.2017.06.005
 
Language en_US
 
Format application/pdf
 
Publisher Elsevier B.V.