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Analysis of rice proteins with DLN repressor Motif/S

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Title Analysis of rice proteins with DLN repressor Motif/S
 
Creator Singh, Purnima
Mathew, Iny Elizebeth
Verma, Ankit
Tyagi, Akhilesh K.
Agarwal, Pinky
 
Subject EAR motif
rice
seed development
trans-repression
yeast
 
Description Accepted date: 31 January 2019
Transcriptional regulation includes both activation and repression of downstream genes.
In plants, a well-established class of repressors are proteins with an ERF-associated amphiphilic
repression/EAR domain. They contain either DLNxxP or LxLxL as the identifying hexapeptide
motif. In rice (Oryza sativa), we have identified a total of 266 DLN repressor proteins, with the
former motif and its modifications thereof comprising 227 transcription factors and 39 transcriptional
regulators. Apart from DLNxxP motif conservation, DLNxP and DLNxxxP motifs with variable
numbers/positions of proline and those without any proline conservation have been identified. Most
of the DLN repressome proteins have a single DLN motif, with higher relative percentage in the
C-terminal region. We have designed a simple yeast-based experiment wherein a DLN motif can
successfully cause strong repression of downstream reporter genes, when fused to a transcriptional
activator of rice or yeast. The DLN hexapeptide motif is essential for repression, and at least two
“DLN” residues cause maximal repression. Comparatively, rice has more DLN repressor encoding
genes than Arabidopsis, and DLNSPP motif from rice is 40% stronger than the known Arabidopsis
SRDX motif. The study reports a straightforward assay to analyze repressor activity, along with the
identification of a strong DLN repressor from rice.
I.E.M. and A.V. acknowledge the junior and senior research fellowships from University
Grants Commission, India and Department of Biotechnology, India, respectively. P.S., A.K.T., and P.A.
acknowledge the research grants from the Department of Biotechnology, India. P.A. is thankful for the core
grant from NIPGR.
 
Date 2019-04-03T07:44:28Z
2019-04-03T07:44:28Z
2019
 
Type Article
 
Identifier International Journal of Molecular Sciences, 20(7): 1600
1422-0067
http://223.31.159.10:8080/jspui/handle/123456789/937
https://www.mdpi.com/1422-0067/20/7/1600/htm
10.3390/ijms20071600
 
Language en_US
 
Format application/pdf
 
Publisher MDPI AG