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Polyamine induction in postharvest banana fruits in response to NO donor SNP occurs via L-arginine mediated pathway and not via competitive diversion of S-adenosyl-L-methionine

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Title Polyamine induction in postharvest banana fruits in response to NO donor SNP occurs via L-arginine mediated pathway and not via competitive diversion of S-adenosyl-L-methionine
 
Creator Lokesh, Veeresh
Manjunatha, Girigowda
Hegde, Namratha S.
Bulle, Mallesham
Puthusseri, Bijesh
Gupta, Kapuganti Jagadis
Neelwarne, Bhagyalakshmi
 
Subject fruit ripening
ethylene
SAM decarboxylase
arginine decarboxylase
ornithine decarboxylase
Musa
 
Description Accepted date: 22 August 2019
Nitric oxide (NO) is known to antagonize ethylene by various mechanisms; one of such mechanisms is reducing ethylene levels by competitive action on S-adenosyl-L-methionine (SAM)—a common precursor for both ethylene and polyamines (PAs) biosynthesis. In order to investigate whether this mechanism of SAM pool diversion by NO occur towards PAs biosynthesis in banana, we studied the effect of NO on alterations in the levels of PAs, which in turn modulate ethylene levels during ripening. In response to NO donor sodium nitroprusside (SNP) treatment, all three major PAs viz. putrescine, spermidine and spermine were induced in control as well as ethylene pre-treated banana fruits. However, the gene expression studies in two popular banana varieties of diverse genomes, Nanjanagudu rasabale (NR; AAB genome) and Cavendish (CAV; AAA genome) revealed the downregulation of SAM decarboxylase, an intermediate gene involved in ethylene and PA pathway after the fifth day of NO donor SNP treatment, suggesting that ethylene and PA pathways do not compete for SAM. Interestingly, arginine decarboxylase belonging to arginine-mediated route of PA biosynthesis was upregulated several folds in response to the SNP treatment. These observations revealed that NO induces PAs via l-arginine-mediated route and not via diversion of SAM pool.
Authors V.L., N.S.H. and B.P. thank CSIR, Govt. of India for the research fellowships. G.M.
thanks DBT, Govt. of India for the research fellowship. Authors thank Pooja Singh and Aprajita Kumari for help
in NO measurements.
 
Date 2019-09-09T07:04:54Z
2019-09-09T07:04:54Z
2019
 
Type Article
 
Identifier Antioxidants (Basel), 8(9): 358
2076-3921
http://223.31.159.10:8080/jspui/handle/123456789/978
https://www.mdpi.com/2076-3921/8/9/358/htm
https://doi.org/10.3390/antiox8090358
 
Language en_US
 
Format application/pdf
 
Publisher MDPI AG