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Absence of correlation between chimeric RNA and aging

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Field Value
 
Title Absence of correlation between chimeric RNA and aging
 
Creator Huang, Reyna
Kumar, Shailesh
Li, Hui
 
Subject chimeric RNA
aging
cis-splicing of adjacent genes
trans-splicing
 
Description Accepted date: 7 December 2017
Chimeric RNAs have been recognized as a phenomenon not unique to cancer cells. They
also exist in normal physiology. Aging is often characterized by deregulation of molecular and
cellular mechanisms, including loss of heterochromatin, increased transcriptional noise, less tight
control on alternative splicing, and more stress-induced changes. It is thus assumed that chimeric
RNAs are more abundant in older people. In this study, we conducted a preliminary investigation
to identify any chimeric RNAs with age-based trends in their expression levels in blood samples.
A chimeric RNA candidate list generated by bioinformatic analysis indicated the possibility of both
negative and positive trends in the expression of chimeric RNAs. Out of this candidate list, five novel
chimeric RNAs were successfully amplified in multiple blood samples and then sequenced. Although
primary smaller sample sizes displayed some weak trends with respect to age, analysis of quantitative
PCR data from larger sample sizes showed essentially no relationship between expression levels and
age. Altogether, these results indicate that, contradictory to the common assumption, chimeric RNAs
as a group are not all higher in older individuals and that placing chimeric RNAs in the context of
aging will be a much more complex task than initially anticipated.
Hui Li is supported by NCI grant CA190713, St. Baldrick’s V Scholar grant, and a Research Scholar Grant (126405-RSG-14-065-01-RMC) from the American Cancer Society.
 
Date 2017-12-18T07:00:26Z
2017-12-18T07:00:26Z
2017
 
Type Article
 
Identifier Genes, 8(12): 386
2073-4425
http://223.31.159.10:8080/jspui/handle/123456789/808
http://www.mdpi.com/2073-4425/8/12/386
10.3390/genes8120386
 
Language en_US
 
Format application/pdf
 
Publisher MDPI AG