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Study on aquaporins of Setaria italica suggests the involvement of SiPIP3;1 and SiSIP1;1 in abiotic stress response

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Title Study on aquaporins of Setaria italica suggests the involvement of SiPIP3;1 and SiSIP1;1 in abiotic stress response
 
Creator Singh, Roshan Kumar
Shweta, Shweta
Muthamilarasan, Mehanathan
Rani, Rekha
Prasad, Manoj
 
Subject Aquaporins
Plasma membrane intrinsic proteins (PIPs)
Tonoplast intrinsic proteins (TIPs)
NOD26-like intrinsic proteins (NIPs)
Small basic intrinsic proteins (SIPs)
Foxtail millet (Setaria italica)
 
Description Accepted date: 26 December 2018
Aquaporins are versatile proteins involved in several biological as well as molecular functions, and they have been extensively
studied in various plant systems. Increasing evidences indicate their role in biotic and abiotic stresses, and therefore, studying
these proteins in a naturally stress-tolerant crop would provide further insights into the roles of this important protein family.
Given this, the present study was performed in foxtail millet (Setaria italica), a model plant for studying biofuel, stress tolerance,
and C4 photosynthetic traits. The study identified 12 plasma membrane intrinsic proteins (PIPs), 11 tonoplast intrinsic proteins
(TIPs), 13 NOD26-like intrinsic proteins (NIPs), and 3 small basic intrinsic proteins (SIPs) in foxtail millet. The identified
proteins and their corresponding genes were characterized using in silico approaches such as chromosomal localization, analysis
of gene and protein properties, phylogenetic analysis, promoter analysis, and RNA-seq-derived expression profiling. The candidate genes identified through these analyses were studied for their expression in response to abiotic stresses (dehydration,
salinity, and heat) as well as hormone treatments (abscisic acid, methyl jasmonate, and salicylic acid) in two contrasting cultivars
of foxtail millet. The study showed that SiPIP3;1 and SiSIP1;1 were differentially expressed in both the cultivars in response to
stress and hormone treatments. Overexpression of these genes in a heterologous yeast system also demonstrated that the
transgenic cells were able to tolerate dehydration as well as salt stress which suggests the involvement of these proteins in the
tolerance mechanism. Overall, the present study provides insights into structure and organization of the aquaporin gene family in
foxtail millet and highlights the potential candidate genes for further functional characterizations.
RKS and RR acknowledge the Council of Scientific
and Industrial Research and Department of Biotechnology, Government
of India, India, respectively, for providing Research Fellowships. SS acknowledges the National Post-Doctoral Fellowship received from DSTSERB, Government of India, India. MM acknowledges the DST
INSPIRE Faculty Award from Department of Science & Technology,
Government of India, India. The authors are thankful to DBT-eLibrary
Consortium (DeLCON) for providing access to the e-resources.
 
Date 2019-02-18T07:00:36Z
2019-02-18T07:00:36Z
2019
 
Type Article
 
Identifier Functional & Integrative Genomics, 19: 587-596
1438-7948
http://223.31.159.10:8080/jspui/handle/123456789/918
https://link.springer.com/article/10.1007%2Fs10142-018-00653-0
https://doi.org/10.1007/s10142-018-00653-0
 
Language en_US
 
Format application/pdf
 
Publisher Springer Nature