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Metabolite signatures of grasspea suspension-cultured cells illustrate the complexity of dehydration response

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Title Metabolite signatures of grasspea suspension-cultured cells illustrate the complexity of dehydration response
 
Creator Rathi, Divya
Pareek, Akanksha
Zhang, Tong
Pang, Qiuying
Chen, Sixue
Chakraborty, Subhra
Chakraborty, Niranjan
 
Subject Dehydration
Hardy legume
Metabolite signature
Non-targeted metabolomics
Suspension culture
Underutilized species
 
Description Accepted date: 6 June 2019
Grasspea, being a hardy legume, is an ideal model system to study stress tolerance mechanisms in plants. In this
study, we investigated the dehydration-responsive metabolome in grasspea suspension-cultured cells (SCCs) to identify the
unique and shared metabolites crucial in imparting dehydration tolerance. To reveal the dehydration-induced metabolite
signatures, SCCs of grasspea were exposed to 10% PEG, followed by metabolomic profling. Chromatographic separation
by HPLC coupled with MRM-MS led to the identifcation of 330 metabolites, designated dehydration-responsive metabolites (DRMs), which belonged to 28 varied functional classes. The metabolome was found to be constituted by carboxylic
acids (17%), amino acids (13.5%), favonoids (10.9%) and plant growth regulators (10%), among others. Pathway enrichment analysis revealed predominance of metabolites involved in phytohormone biosynthesis, secondary metabolism and
osmotic adjustment. Exogenous application of DRMs, arbutin and acetylcholine, displayed improved physiological status in
stress-resilient grasspea as well as hypersensitive pea, while administration of lauric acid imparted detrimental efects. This
represents the frst report on stress-induced metabolomic landscape of a crop species via a suspension culture system, which
would provide new insights into the molecular mechanism of stress responses and adaptation in crop species.
We thank the Council of Scientifc and Industrial
Research (CSIR), Govt. of India, for providing pre-doctoral fellowship
to D.R. and the University Grant Commission (UGC), Govt. of India,
for providing pre-doctoral fellowship to A.P. This work was supported
by Grants (38/1385/14/EMR-II) from the Council of Scientifc and
Industrial Research (CSIR), Govt. of India.
 
Date 2019-06-18T05:29:56Z
2019-06-18T05:29:56Z
2019
 
Type Article
 
Identifier Planta, 250(3): 857-871
1432-2048
http://223.31.159.10:8080/jspui/handle/123456789/957
https://link.springer.com/article/10.1007%2Fs00425-019-03211-5
https://doi.org/10.1007/s00425-019-03211-5
 
Language en_US
 
Format application/pdf
 
Publisher Springer Nature Publishing AG