Production and cytological characterization of a synthetic amphiploid derived from a cross between Oryza sativa and Oryza punctata
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Title |
Production and cytological characterization of a synthetic amphiploid derived from a cross between Oryza sativa and Oryza punctata
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Creator |
Kumar, Kishor
Neelam, Kumari Singh, Gurpreet Mathan, Jyotirmaya Ranjan, Aashish Brar, Darshan Singh Singh, Kuldeep |
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Subject |
Oryza punctata
synthetic amphiploids aneuploids cytology flow cytometery chromosome doubling |
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Description |
Accepted date: 9 July 2019
Oryza punctata Kotschy ex Steud. (BB, 2n = 24) is a wild species of rice that has many useful agronomic traits. An interspecific hybrid (AB, 2n = 24) was produced by crossing O. punctata and Oryza sativa variety Punjab Rice 122 (PR122, AA, 2n = 24) to broaden the narrow genetic base of cultivated rice. Cytological analysis of the pollen mother cells (PMCs) of the interspecific hybrids confirmed that they have 24 chromosomes. The F1 hybrids showed the presence of 19–20 univalents and 1–3 bivalents. The interspecific hybrid was treated with colchicine to produce a synthetic amphiploid (AABB, 2n = 48). Pollen fertility of the synthetic amphiploid was found to be greater than 50% and partial seed set was observed. Chromosome numbers in the PMCs of the synthetic amphiploid were 24II, showing normal pairing. Flow cytometric analysis also confirmed doubled genomic content in the synthetic amphiploid. Leaf morphological and anatomical studies of the synthetic amphiploid showed higher chlorophyll content and enlarged bundle sheath cells as compared with both of its parents. The synthetic amphiploid was backcrossed with PR122 to develop a series of addition and substitution lines for the transfer of useful genes from O. punctata with least linkage drag. The authors are thankful to the International Rice Research Institute, Philippines, Manila for sharing wild species germplasm of rice. Authors also thank the Indian Council of Agricultural Research (ICAR), New Delhi funded project “Niche Area of Excellence in Wheat and Rice, PC 2198” for providing a financial grant. |
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Date |
2019-12-19T10:34:27Z
2019-12-19T10:34:27Z 2019 |
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Type |
Article
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Identifier |
Genome 62(11): 705-714
1480-3321 http://223.31.159.10:8080/jspui/handle/123456789/1026 https://www.nrcresearchpress.com/doi/full/10.1139/gen-2019-0062?url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&rfr_dat=cr_pub%3Dpubmed&#.XftFGWQzaUl https://doi.org/10.1139/gen-2019-0062 |
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Language |
en_US
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Format |
application/pdf
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Publisher |
NRC Research Press
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