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Production and cytological characterization of a synthetic amphiploid derived from a cross between Oryza sativa and Oryza punctata

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Title Production and cytological characterization of a synthetic amphiploid derived from a cross between Oryza sativa and Oryza punctata
 
Creator Kumar, Kishor
Neelam, Kumari
Singh, Gurpreet
Mathan, Jyotirmaya
Ranjan, Aashish
Brar, Darshan Singh
Singh, Kuldeep
 
Subject Oryza punctata
synthetic amphiploids
aneuploids
cytology
flow cytometery
chromosome doubling
 
Description Accepted date: 9 July 2019
Oryza punctata Kotschy ex Steud. (BB, 2n = 24) is a wild species of rice that has many useful agronomic
traits. An interspecific hybrid (AB, 2n = 24) was produced by crossing O. punctata and Oryza sativa variety Punjab Rice
122 (PR122, AA, 2n = 24) to broaden the narrow genetic base of cultivated rice. Cytological analysis of the pollen
mother cells (PMCs) of the interspecific hybrids confirmed that they have 24 chromosomes. The F1 hybrids showed
the presence of 19–20 univalents and 1–3 bivalents. The interspecific hybrid was treated with colchicine to produce
a synthetic amphiploid (AABB, 2n = 48). Pollen fertility of the synthetic amphiploid was found to be greater than
50% and partial seed set was observed. Chromosome numbers in the PMCs of the synthetic amphiploid were 24II,
showing normal pairing. Flow cytometric analysis also confirmed doubled genomic content in the synthetic
amphiploid. Leaf morphological and anatomical studies of the synthetic amphiploid showed higher chlorophyll
content and enlarged bundle sheath cells as compared with both of its parents. The synthetic amphiploid was
backcrossed with PR122 to develop a series of addition and substitution lines for the transfer of useful genes from
O. punctata with least linkage drag.
The authors are thankful to the International Rice Research Institute, Philippines, Manila for sharing wild species germplasm of rice. Authors also thank the Indian
Council of Agricultural Research (ICAR), New Delhi
funded project “Niche Area of Excellence in Wheat and
Rice, PC 2198” for providing a financial grant.
 
Date 2019-12-19T10:34:27Z
2019-12-19T10:34:27Z
2019
 
Type Article
 
Identifier Genome 62(11): 705-714
1480-3321
http://223.31.159.10:8080/jspui/handle/123456789/1026
https://www.nrcresearchpress.com/doi/full/10.1139/gen-2019-0062?url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&rfr_dat=cr_pub%3Dpubmed&#.XftFGWQzaUl
https://doi.org/10.1139/gen-2019-0062
 
Language en_US
 
Format application/pdf
 
Publisher NRC Research Press