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Expression profiling of miRNAs indicates crosstalk between phytohormonal response and rhizobial infection in chickpea

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Title Expression profiling of miRNAs indicates crosstalk between phytohormonal response and rhizobial infection in chickpea
 
Creator Tiwari, Manish
Bhatia, Sabhyata
 
Subject Auxin
Cytokinin
miRNA
Nodule
qRT-PCR
Rhizobia
 
Description Accepted date: 20 November 2019
Legumes develop root nodules in which bacteria fix nitrogen for plants. The phytohormones auxin and cytokinin regulate
nodule organogenesis by recruiting various genes to effect symbiosis. Moreover, these genes are regulated by the action of
microRNAs also. To understand the complex regulatory network involving miRNAs in response to phytohormones and
rhizobial interactions in chickpea roots, a miRNA expression profiling was performed. Indole acetic acid and 6-benzylaminopurine at concentrations of 0.1, 1 and 10 lM were used for auxin and cytokinin exogenous treatment and Mesorhizobium ciceri to study rhizobial interaction with chickpea root. Expression profiling of a set of 11 miRNAs was
performed. Further, the targets of the candidate miRNAs were identified, followed by functional annotation. This analysis
revealed that cat-miR160, cat-miR164, cat-miR396 and cat-miR398 were responsive to auxin and cytokinin. cat-miR319
was found to be only auxin responsive and is known to regulate auxin signalling by targeting TEOSINTE BRANCHED/
CYCLOIDEA/PCF (TCP) which interacts with auxin inducible genes. Further, cytokinin elicited a response at very low
concentration of 0.1 lM, and most of the miRNAs investigated were responsive to cytokinin. Interactome analysis
revealed that cat-miR164 and cat-miR168 work in conjunction to regulate auxin signalling. Interestingly, cat-miR169 and
cat-miR482 were low expressing during auxin treatment and M. ciceri infection but their expression spiked during
cytokinin treatment, indicating a cytokinin mediated mode of action. The miRNA expression profiling in response to
phytohormones and rhizobia and the reported function of their target genes suggested a crosstalk among the phytohormonal
responses during chickpea nodulation.
This work was supported by National Institute of
Plant Genome Research (NIPGR) and grant (BT/PR3305/AGR/2/816/
2011) from Department of Biotechnology, India (http://www.dbtin
dia.nic.in). We kindly acknowledge the University Grant Commission
(UGC), Govt. of India for providing predoctoral fellowship to Manish
Tiwari.
 
Date 2019-12-17T10:28:54Z
2019-12-17T10:28:54Z
2020
 
Type Article
 
Identifier Journal of Plant Biochemistry and Biotechnology, 29: 380–394
0974-1275
http://223.31.159.10:8080/jspui/handle/123456789/1025
https://link.springer.com/article/10.1007%2Fs13562-019-00545-9
https://doi.org/10.1007/s13562-019-00545-9
 
Language en_US
 
Format application/pdf
 
Publisher Springer Nature Publishing AG