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Complete genome dynamics of a dominant-lineage strain of Xanthomonas oryzae pv. oryzae harbouring a novel plasmid encoding a type IV secretion system

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Title Complete genome dynamics of a dominant-lineage strain of Xanthomonas oryzae pv. oryzae harbouring a novel plasmid encoding a type IV secretion system
 
Creator Kaur, Amandeep
Bansal, Kanika
Kumar, Sanjeet
Sonti, Ramesh V.
Patil, Prabhu B.
 
Subject Xanthomonas
type IV secretion system
monocots
xylem
nanopore sequencing
 
Description Accepted date: 27 August 2019
Xanthomonas oryzae pv. oryzae (Xoo) is a serious pathogen causing bacterial blight disease in rice. Population genomic studies
have revealed that rampant inter-strain rather than inter-lineage differences are contributing to the evolutionary success of
this pathogen. Here, we report the complete genome sequence of BXO1, a strain of Xoo belonging to a dominant lineage from
India. A complete genome-based investigation revealed the presence of two plasmids, pBXO1-1 (66.7kb) and pBXO1-2 (25.6kb).
The pBXO1-1 plasmid encodes 71 genes, 38 of which encode hypothetical proteins of unknown function. However, these hypothetical genes possess atypical GC content, pointing towards their acquisition and movement through horizontal gene transfer.
Interestingly, pBXO1-2 encodes a type IV secretion system (T4SS), which is known to play an important role in the conjugative
transfer of genetic material, and also provides fitness to pathogenic bacteria for their enhanced survival. Neither plasmid has
been reported previously in any other complete Xoo genome published to date. Our analysis also revealed that the pBXO1-2
plasmid is present in Xanthomonas albilineans str. GPE PC73, which is known to cause leaf scald, a lethal disease in sugarcane.
Our complete genome sequence analysis of BXO1 has provided us with detailed insights into the two novel strain-specific plasmids, in addition to decoding their functional capabilities, which were not assessable when using the draft genome sequence
of the strain. Overall, our study has revealed the mobility of a novel T4SS in two pathogenic species of Xanthomonas that infect
the vascular tissues of two economically important monocot plants, i.e. rice and sugarcane.
A. K. is supported by a DST-INSPIRE fellowship. This work is supported
by a project entitled ‘High throughput and integrative genomic
approaches to understand adaptation of probiotic and pathogenic
bacterium’ (OLP148) of the Council of Scientific and Industrial Research
(CSIR) to P.B.P. R. V. S. was also supported by a J. C. Bose fellowship from
the Science and Engineering Research Board, Government of India.
 
Date 2019-09-20T07:49:41Z
2019-09-20T07:49:41Z
2019
 
Type Article
 
Identifier Access Microbiology, 1(9): e000063
2516-8290
http://223.31.159.10:8080/jspui/handle/123456789/985
https://www.microbiologyresearch.org/content/journal/acmi/10.1099/acmi.0.000063
https://doi.org/10.1099/acmi.0.000063
 
Language en_US
 
Format application/pdf
 
Publisher Microbiology Society