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Nitrite and nitric oxide are important in the adjustment of primary metabolism during the hypersensitive response in tobacco

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Title Nitrite and nitric oxide are important in the adjustment of primary metabolism during the hypersensitive response in tobacco
 
Creator Mur, Luis A. J.
Kumari, Aprajita
Brotman, Yariv
Zeier, Jurgen
Mandon, Julien
Cristescu, Simona M.
Harren, Frans
Kaiser, Werner M.
Fernie, Alisdair R.
Gupta, Kapuganti Jagadis
 
Subject Amino acid metabolism
nitrate
nitric oxide
nitrite
nitrite reductase
 
Description Accepted date: 29 March 2019
Nitrate and ammonia deferentially modulate primary metabolism during the hypersensitive response in tobacco. In this study, tobacco RNAi lines with low nitrite reductase (NiRr) levels were used to investigate the roles of nitrite and nitric oxide (NO) in this process. The lines accumulate NO2-, with increased NO generation, but allow sufficient reduction to NH4+ to maintain plant viability. For wild-type (WT) and NiRr plants grown with NO3-, inoculation with the non-host biotrophic pathogen Pseudomonas syringae pv. phaseolicola induced an accumulation of nitrite and NO, together with a hypersensitive response (HR) that resulted in decreased bacterial growth, increased electrolyte leakage, and enhanced pathogen resistance gene expression. These responses were greater with increases in NO or NO2- levels in NiRr plants than in the WT under NO3- nutrition. In contrast, WT and NiRr plants grown with NH4+ exhibited compromised resistance. A metabolomic analysis detected 141 metabolites whose abundance was differentially changed as a result of exposure to the pathogen and in response to accumulation of NO or NO2-. Of these, 13 were involved in primary metabolism and most were linked to amino acid and energy metabolism. HR-associated changes in metabolism that are often linked with primary nitrate assimilation may therefore be influenced by nitrite and NO production.
The authors wish to thank the UKIERI-DST fund and DBT, DSTSERB for partially funding this work. We declare no conflicts of interest
in relation to this work.
 
Date 2019-06-10T08:33:32Z
2019-06-10T08:33:32Z
2019
 
Type Article
 
Identifier Journal of Experimental Botany, 70(17): 4571-4582
1460-2431
http://223.31.159.10:8080/jspui/handle/123456789/956
https://academic.oup.com/jxb/advance-article/doi/10.1093/jxb/erz161/5438283
https://doi.org/10.1093/jxb/erz161
 
Language en_US
 
Format application/pdf
 
Publisher Oxford University Press