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Optimizing in vitro culture conditions for the biotrophic fungi Exobasidium vexans through response surface methodology

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Title Optimizing in vitro culture conditions for the biotrophic fungi Exobasidium vexans through response surface methodology
 
Creator Chaliha, Chayanika
Kalita, Eeshan
Verma, Praveen K.
 
Subject Blister blight
Exobasidium vexans
Growth media
Optimization
RSM
 
Description Accepted date: 3 December 2019
The blister blight disease caused by the fungus,
Exobasidium vexans has serious implications on the quality
of tea production. The disease however, has been poorly
studied and hence there is very limited information on the
pathogen and as such the pathogenesis of blister blight
infection. One of the major roadblocks in understanding E.
vexans is the obligate and biotrophic nature of the fungus
which limits the establishment and maintenance of in vitro
cultures. To address this issue, a Central Composite Design
based Response Surface Methodology (RSM) was adopted
to study the modification of three fungal culture media viz.
czapek dox, potato dextrose, and v8 juice, and the effect of
altered media composition on growth conditions and media
compositions were assessed. The response parameter for
the RSM experiments was the mycelial biomass produced
under different culture conditions. The uni and bi-parametric interactions among the experimental variables provided the basis for the statistically optimized conditions for
maximal fungal growth. The study thus presents the recommended modifications of existing media that can lead to
the successful establishment and maintenance of E. vexans
in vitro cultures.
The authors wish to acknowledge DBT, Govt.
of India, for the Twinning Research Grant (Grant No. BT/427/NE/
TBP/2013). Author C.C. would like to acknowledge DST, Govt. of
India for her DST INSPIRE Junior Research Fellowship (IF-150964).
The authors thank Ananda Tea Estate, Lakhimpur District, Assam for
providing the blister blight infected tea leaf samples used in the study.
 
Date 2019-12-17T04:45:47Z
2019-12-17T04:45:47Z
2020
 
Type Article
 
Identifier Indian Journal of Microbiology, 60(2): 167-174
0973-7715
http://223.31.159.10:8080/jspui/handle/123456789/1022
https://link.springer.com/article/10.1007/s12088-019-00846-6
https://doi.org/10.1007/s12088-019-00846-6
 
Language en_US
 
Format application/pdf
 
Publisher Springer Nature Publishing AG