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FCS-like zinc finger 6 and 10 repress SnRK1 signalling in Arabidopsis

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Title FCS-like zinc finger 6 and 10 repress SnRK1 signalling in Arabidopsis
 
Creator Jamsheer, Muhammed K.
Sharma, Manvi
Singh, Dhriti
Mannully, Chanchal Thomas
Jindal, Sunita
Shukla, Brihaspati Narayan
Laxmi, Ashverya
 
Subject FCS-Like Zinc Finger
Arabidopsis
SNF1-Related Protein Kinase 1 (SnRK1)
 
Description Accepted date: February 6, 2018
SNF1-Related Protein Kinase 1 (SnRK1) is a central regulator of plant growth during energy starvation. The FCS-Like Zinc finger (FLZ) proteins are recently identified adaptor proteins which facilitate the interaction of SnRK1 with other proteins. In this study, we identified that two starvation-induced FLZ genes, FLZ6 and FLZ10, work as repressors of SnRK1 signalling. The reduced expression of these genes resulted in an increase in the level of SnRK1α1, which is the major catalytic subunit of SnRK1. This resulted in a concomitant increase in the phosphorylated protein and SnRK1 activity in the flz6 and flz10 mutants. FLZ6 and FLZ10 specifically interact with SnRK1α subunits in the cytoplasmic foci which colocalized with the endoplasmic reticulum. In physiological assays, similar to SnRK1α1 overexpression line, flz mutants showed compromised growth. Further, the growth promotion in response to favourable growth conditions was found to be attenuated in the mutants. The enhanced SnRK1 activity in the mutants resulted in a reduction in the level of phosphorylated RIBOSOMAL S6 KINASE and the expression of E2Fa and its targets indicating that TARGET OF RAPAMYCIN-dependent promotion of protein synthesis and cell cycle progression is impaired. Taken together, this study uncovers a plant-specific modulation of SnRK1 signalling.
 
Date 2018-01-23T09:23:20Z
2018-01-23T09:23:20Z
2018
 
Type Article
 
Identifier Plant Journal, 94(2): 232-245
1365-313X
http://223.31.159.10:8080/jspui/handle/123456789/830
http://onlinelibrary.wiley.com/doi/10.1111/tpj.13854/abstract;jsessionid=2FB748513B36F071BA42DD4FA4CB8401.f02t02
 
Language en_US
 
Format application/pdf
 
Publisher John Wiley & Sons