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Dehydration-responsive alterations in the chloroplast proteome and cell metabolomic profile of rice reveals key stress adaptation responses

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Title Dehydration-responsive alterations in the chloroplast proteome and cell metabolomic profile of rice reveals key stress adaptation responses
 
Creator Gayen, Dipak
Barua, Pragya
Lande, Nilesh Vikram
Varshney, Swati
Sengupta, Shantanu
Chakraborty, Subhra
Chakraborty, Niranjan
 
Subject Adaptive responses
Cereal crop
Dehydration response
Organellar proteome
Untargeted metabolome
Photosynthesis
Semi-autonomous organelle
Water-use efficiency
 
Description Accepted: 9 January 2019
Chloroplast is a semi-autonomous organelle in plants and other photosynthetic eukaryotes, playing a fundamental role of regulating photosynthesis. It is also responsible for sustaining essential biosynthetic reactions
including synthesis of amino acids, fatty acids and terpenes. Photosynthesis, the conversion of light energy into
chemical energy, serves as the sensor of environmental changes and augments different cellular functions to
initiate adaptive responses. However, the molecular processes and regulatory mechanisms of dehydration tolerance adopted by chloroplast remain largely unknown. To gain a better understanding of dehydration response,
a chloroplast proteome map of rice was developed. Four-week-old rice seedlings were subjected to dehydration
by withholding water for 9 d, and the magnitude of dehydration-induced damage to the chloroplast was
monitored. The iTRAQ-based quantitative proteome analysis led to the identification of 40 differentially regulated proteins (DRPs). The DRPs were presumably involved in a wide array of metabolic processes including
chloroplast energy metabolism, photosynthesis and defense response. Furthermore, dehydration-induced
changes in the metabolite profile and network analysis revealed a high abundance of branched chain amino
acids and sugar that might reduce osmotic potential, thereby protecting cellular integrity. The proteomics approach revealed altered status of major photosynthesis related proteins, while cell metabolite profile demonstrated alteration of tricarboxylic acid cycle intermediates, indicating dehydration-triggered alterations in ATP
production and energy metabolism. Altogether, these results demonstrated that the global regulation of chloroplast proteome is intimately linked to cellular metabolic rewiring of adaptive responses, which may favor
genetic manipulation of crop species for better adaptation.
This work was supported by grants from the Department of
Biotechnology (DBT), India (BT/AGR/CG-PhaseII/01/2014) and
National Institute of Plant Genome Research, New Delhi to N.C. We
thank Department of Science and Technology (DST), India for SERBNational Post-Doctoral Fellowship (PDF/2016/002615) granted to D.G.
and Department of Biotechnology (DBT) for providing pre-doctoral
fellowship to P.B. We also acknowledge Council of Scientific &
Industrial Research (CSIR), India for providing pre-doctoral fellowship
to N.V.L. We thank Mr. Jasbeer Singh for manuscript layout and format.
 
Date 2019-02-11T06:07:54Z
2019-02-11T06:07:54Z
2019
 
Type Article
 
Identifier Environmental and Experimental Botany, 160: 12-24
0098-8472
http://223.31.159.10:8080/jspui/handle/123456789/917
https://www.sciencedirect.com/science/article/pii/S0098847218316514
https://doi.org/10.1016/j.envexpbot.2019.01.003
 
Language en_US
 
Format application/pdf
 
Publisher Elsevier B.V.