Record Details

Analysis of genes encoding seed storage proteins (SSPs) in chickpea (Cicer arietinum L.) reveals co-expressing transcription factors and a seed-specific promoter

NIPGR Digital Knowledge Repository (NDKR)

View Archive Info
 
 
Field Value
 
Title Analysis of genes encoding seed storage proteins (SSPs) in chickpea (Cicer arietinum L.) reveals co-expressing transcription factors and a seed-specific promoter
 
Creator Verma, Subodh
Bhatia, Sabhyata
 
Subject Seed storage proteins (SSPs)
Promoter analysis
Co-expression analysis
Chickpea
 
Description Accepted date: 26 November 2018
Improvement of the quality and quantity of chickpea seed protein can be greatly facilitated by an understanding of the genic
organization and the genetic architecture of the genes encoding seed storage proteins (SSPs). The aim of this study was to provide
a comprehensive analysis of the chickpea SSP genes, putative co-expressing transcription factors (TFs), and to identify a seedspecific SSP gene promoter. A genome-wide identification of SSP genes in chickpea led to the identification of 21 non-redundant
SSP encoding genes located on 6 chromosomes. Phylogenetic analysis grouped SSP genes into 3 subgroups where members
within the same clade demonstrated similar motif composition and intron-exon organization. Tandem duplications were identified to be the major contributors to the expansion of the SSP gene family in chickpea. Co-expression analysis revealed 14 TFs
having expression profiles similar to the SSP genes that included members of important TF families that are known to regulate
seed development. Expression analysis of SSP genes and TFs revealed significantly higher expression in late stages of seed
development as well as in high seed protein content (HPC) genotypes. In silico analysis of the promoter regions of the SSP
encoding genes revealed several seed-specific cis-regulatory elements such as RY repeats, ACGT motifs, CAANTG, and GCN4.
A candidate promoter was analyzed for seed specificity by generating stable transgenics in Arabidopsis. Overall, this study
provides a useful resource to explore the regulatory networks involved in SSP synthesis and/or accumulation for utilization in
developing nutritionally improved chickpea genotypes.
SV acknowledges the award of research fellowship
from the Department of Biotechnology, Govt. of India. Authors thank
Mr. Sandeep Yadav and Dr. Anand Sarkar for helping in microscopy,
and Dr. Chandra Kant for helping in in silico expression profiling.
Authors thank Dr. Swarup K Parida for the kind gift of HPC, LPC chickpea accessions. The authors are thankful to DBT-eLibrary Consortium
(DeLCON) for providing access to e-resources.
 
Date 2018-12-20T06:36:57Z
2018-12-20T06:36:57Z
2019
 
Type Article
 
Identifier Functional & Integrative Genomics, 19(3): 373-390
1438-7948
http://223.31.159.10:8080/jspui/handle/123456789/904
https://link.springer.com/article/10.1007%2Fs10142-018-0650-8
https://doi.org/10.1007/s10142-018-0650-8
 
Language en_US
 
Format application/pdf
 
Publisher Springer Nature