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Mediator subunit OsMED14_1 plays an important role in rice development

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Title Mediator subunit OsMED14_1 plays an important role in rice development
 
Creator Malik, Naveen
Ranjan, Rajeev
Parida, Swarup K.
Agarwal, Pinky
Tyagi, Akhilesh K.
 
Subject Auxin
cell-cycle
development
expression
interaction
Mediator
rice
 
Description Accepted date: 8 November 2019
Mediator, a multi‐subunit coactivator complex, regulates transcription in eukaryotes and is involved in diverse processes in Arabidopsis through its different subunits. Here, we have explored developmental aspects of one of the rice Mediator subunit gene OsMED14_1. We analyzed its expression pattern through RNA in‐situ hybridization and pOsMED14_1:GUS transgenics which showed its expression in roots, leaves, anthers and seeds prominently at younger stages, indicating possible involvement of this subunit in multiple aspects of rice development. To understand developmental roles of OsMED14_1 in rice, we generated and studied RNAi based knockdown rice plants which showed multiple effects including less height, narrower leaves and culms with reduced vasculature, lesser lateral root branching, defective microspore development, reduced panicle branching and seed set, and smaller seeds. Histological analyses showed that slender organs were caused by reduction in both cell number and cell size in OsMED14_1 knockdown plants. Flow cytometric analyses and expression analyses of cell‐cycle related genes revealed that defective cell‐cycle progression led to these defects. Expression analyses of auxin related genes and IAA immuno‐localization study indicated altered auxin level in these knockdown plants. Reduction of lateral root branching in knockdown plants was corrected by exogenous IAA supplement. OsMED14_1 physically interacts with transcription factors YABBY5, TDR and MADS29, possibly regulating auxin homeostasis and ultimately leading to lateral organ/leaf, microspore and seed development.
The authors acknowledge the financial support provided by NIPGR, New Delhi and Department
of Biotechnology (DBT), Government of India for conducting the study. NM and RR appreciate
Council of Scientific and Industrial Research (CSIR), India for providing Junior and Senior
Research Fellowships and NIPGR for Senior Research Fellowship. Authors also convey thanks to
Central Instrument Facility (CIF) and Plant Growth Facility (PGF) of NIPGR, New Delhi for
providing timely access to instruments and other resources for the research work.
 
Date 2019-11-14T11:33:26Z
2019-11-14T11:33:26Z
2020
 
Type Article
 
Identifier Plant Journal, 101: 1411-1429
1365-313X
http://223.31.159.10:8080/jspui/handle/123456789/1013
https://onlinelibrary.wiley.com/doi/abs/10.1111/tpj.14605
https://doi.org/10.1111/tpj.14605
 
Language en_US
 
Format application/pdf
 
Publisher John Wiley & Sons