ICAR Research Data Repository for Knowledge Management
<p style="text-align: justify;">Cytotoxic effect of methanolic extracts and partially purified fractions of some medicinal plants used in traditional medication</p>
Online Publishing @ NISCAIR
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| Authentication Code |
dc |
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| Title Statement |
<p style="text-align: justify;">Cytotoxic effect of methanolic extracts and partially purified fractions of some medicinal plants used in traditional medication</p> |
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| Added Entry - Uncontrolled Name |
Badgujar, Nutan Vinay; Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied Sciences (ARIBAS), affiliated to Sardar Patel University, Vallabh Vidyanagar, Gujarat 388120, India Mistry, Kinnari N; Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied Sciences (ARIBAS), affiliated to Sardar Patel University, Vallabh Vidyanagar, Gujarat 388120, India Rank, Dharamshibhai N; Department of Animal Breeding and Genetics, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, 388110, India Joshi, Chaitanya G; Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, 388110, India |
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| Uncontrolled Index Term |
Apoptosis; Cytotoxic activity; DNA fragmentation assay; MTT assay; Real-time PCR assay. |
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| Summary, etc. |
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</style> <![endif]--><span style="font-size: 9.0pt; font-family: "Times New Roman","serif"; mso-fareast-font-family: "Times New Roman"; color: black; mso-themecolor: text1; mso-ansi-language: EN-GB; mso-fareast-language: EN-US; mso-bidi-language: AR-SA; mso-bidi-font-weight: bold;" lang="EN-GB">In this study, the cytotoxic activity of methanolic extracts different parts of seven plant species was checked on NRK-52E (Rat renal proximal tubular cells) using MTT assay. Based on their cytotoxic activities, methanol extract of <em style="mso-bidi-font-style: normal;">Vitex negundo (V. negundo)</em> was selected and their partition in hexane, chloroform, ethylacetate, butanol and water was done. Among all fractions, chloroform fraction was most active on NRK-52E cells as determined by MTT assay. In NRK-52E cells induction of apoptosis was checked by analyzing DNA fragmentation by agarose gel electrophoresis. To study the molecular mechanism of apoptosis, expression levels of different genes BCL-2, BCL-Xl, SOD, TGF, Foxo and BAX were assessed using quantitative real-time PCR. Chloroform fraction of <em style="mso-bidi-font-style: normal;">Vitex negundo </em>(VnCE) was found to be highly antiproliferative and also showed DNA fragmentation in NRK-52E cells. VnCE showed up regulation of BCL-2, BCL-Xl, SOD, Foxo and BAX genes and down regulation of TGF gene.</span></p><div id="_mcePaste" class="mcePaste" style="position: absolute; left: -10000px; top: 0px; width: 1px; height: 1px; overflow: hidden;"><p class="MsoNormal" style="margin-bottom: 0.0001pt; text-align: justify; line-height: 200%; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial; vertical-align: baseline;"><span style="font-size: 9.0pt; line-height: 200%; font-family: "Times New Roman","serif";" lang="EN-IN">Cytotoxic activity, Apoptosis, DNA fragmentation assay, MTT assay, Real time PCR assay</span></p></div> |
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| Publication, Distribution, Etc. |
Indian Journal of Natural Products and Resources (IJNPR) [Formerly Natural Product Radiance (NPR)] 2020-01-01 10:58:09 |
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| Electronic Location and Access |
application/pdf http://op.niscair.res.in/index.php/IJNPR/article/view/18338 |
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| Data Source Entry |
Indian Journal of Natural Products and Resources (IJNPR) [Formerly Natural Product Radiance (NPR)]; ##issue.vol## 10, ##issue.no## 2 (2019): June, 2019 |
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| Language Note |
en |
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