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Quantitative phosphoproteomic analysis of legume using TiO2-based enrichment coupled with isobaric labeling

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Title Quantitative phosphoproteomic analysis of legume using TiO2-based enrichment coupled with isobaric labeling
 
Creator Barua, Pragya
Lande, Nilesh Vikram
Kumar, Sunil
Chakraborty, Subhra
Chakraborty, Niranjan
 
Subject False discovery rate
Legumes
Nuclear fraction
Phosphopeptide enrichment
Phosphoproteome
Protein phosphorylation
Stress response
Titanium dioxide
 
Description Accepted date: 1 Jan 2020
Phosphorylation of proteins is the most dynamic protein modification, and its analysis aids in determining the functional and regulatory principles of important cellular pathways. The legumes constitute the third largest family of higher plants, Fabaceae, comprising about 20,000 species and are second to cereals in agricultural importance on the basis of global production. Therefore, an understanding of the developmental and adaptive processes of legumes demands identification of their regulatory components. The most crucial signature of the legume family is the symbiotic nitrogen fixation, which makes this fascinating and interesting to investigate phosphorylation events. The research on protein phosphorylation in legumes has been focused primarily on two model species, Medicago truncatula and Lotus japonicus. The development of reciprocal research in other species, particularly the crops, is lagging behind which has limited its beneficial uses in agricultural productivity. In this chapter, we outline the titanium dioxide-based enrichment of phosphopeptides for nuclear proteome analysis of a grain legume, chickpea.
This work was supported by grants from the Department of Science
and Technology (DST)-SERB (EMR/2015/001870), India. The
authors thank Department of Biotechnology (DBT) and Council
of Scientific and Industrial Research (CSIR), India for providing
research fellowship to PB, NVL, and SK, respectively.
 
Date 2020-01-07T06:09:58Z
2020-01-07T06:09:58Z
2020
 
Type Article
 
Identifier Methods in Molecular Biology, 2107: 395-406
978-1-0716-0235-5
http://223.31.159.10:8080/jspui/handle/123456789/1028
 
Language en_US
 
Format application/pdf
 
Publisher Springer Nature Publishing AG