Quantitative phosphoproteomic analysis of legume using TiO2-based enrichment coupled with isobaric labeling
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Title |
Quantitative phosphoproteomic analysis of legume using TiO2-based enrichment coupled with isobaric labeling
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Creator |
Barua, Pragya
Lande, Nilesh Vikram Kumar, Sunil Chakraborty, Subhra Chakraborty, Niranjan |
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Subject |
False discovery rate
Legumes Nuclear fraction Phosphopeptide enrichment Phosphoproteome Protein phosphorylation Stress response Titanium dioxide |
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Description |
Accepted date: 1 Jan 2020
Phosphorylation of proteins is the most dynamic protein modification, and its analysis aids in determining the functional and regulatory principles of important cellular pathways. The legumes constitute the third largest family of higher plants, Fabaceae, comprising about 20,000 species and are second to cereals in agricultural importance on the basis of global production. Therefore, an understanding of the developmental and adaptive processes of legumes demands identification of their regulatory components. The most crucial signature of the legume family is the symbiotic nitrogen fixation, which makes this fascinating and interesting to investigate phosphorylation events. The research on protein phosphorylation in legumes has been focused primarily on two model species, Medicago truncatula and Lotus japonicus. The development of reciprocal research in other species, particularly the crops, is lagging behind which has limited its beneficial uses in agricultural productivity. In this chapter, we outline the titanium dioxide-based enrichment of phosphopeptides for nuclear proteome analysis of a grain legume, chickpea. This work was supported by grants from the Department of Science and Technology (DST)-SERB (EMR/2015/001870), India. The authors thank Department of Biotechnology (DBT) and Council of Scientific and Industrial Research (CSIR), India for providing research fellowship to PB, NVL, and SK, respectively. |
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Date |
2020-01-07T06:09:58Z
2020-01-07T06:09:58Z 2020 |
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Type |
Article
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Identifier |
Methods in Molecular Biology, 2107: 395-406
978-1-0716-0235-5 http://223.31.159.10:8080/jspui/handle/123456789/1028 |
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Language |
en_US
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Format |
application/pdf
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Publisher |
Springer Nature Publishing AG
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