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Novel insights into expansion and functional diversification of MIR169 family in tomato

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Title Novel insights into expansion and functional diversification of MIR169 family in tomato
 
Creator Rao, Sombir
Balyan, Sonia
Jha, Sarita
Mathur, Saloni
 
Subject Abiotic stress
Degradome
Duplication
Novel targets
Polycistronic
Sly-MIR169
Short-tandem-target-mimic
Tomato
 
Description Accepted date: 13 January 2020
MIR169 family is an evolutionarily conserved miRNA family in plants. A systematic in-depth analysis of MIR169 family in tomato is lacking. We report 18 miR169 precursors, annotating new loci for MIR169a, b and d, as well as 3 novel mature isoforms (MIR169f/g/h). The family has expanded by both tandem- and segmental-duplication events during evolution. A tandem-pair MIR169b/b-1 and MIR169b-2/h is polycistronic in nature coding for three MIR169b isoforms and a new variant miR169h, that is evidently absent in the wild relatives S. pennellii and S. pimpinellifolium. Seven novel miR169 targets including RNA-binding protein, protein-phosphatase, aminotransferase, chaperone, tetratricopeptide-repeat-protein, and transcription factors ARF-9B and SEPELLATA-3 were established by efficient target cleavage in the presence of specific precursors as well as increased target abundance upon miR169 chelation by short-tandem-target-mimic construct in transient assays. Comparative antagonistic expression profiles of MIR169:target pairs suggest MIR169 family as ubiquitous regulator of various abiotic stresses (heat, cold, dehydration and salt) and developmental pathways. This regulation is partly brought about by acquisition of new promoters as demonstrated by promoter MIR169:GUS reporter assays as well as differential processivity of different precursors and miRNA cleavage efficiencies. Thus, the current study augments the functional horizon of MIR169 family with applications for stress tolerance in crops.
The authors acknowledge NIPGR core grant, phytotron facility, CIF and field area. CLN1621L seeds were provided by AVRDC, TAIWAN. SR and SJ acknowledge Department of Biotechnology (DBT) and University Grants Commission (UGC) Govt. of India, respectively, for the award of research fellowships.
 
Date 2020-01-28T05:00:23Z
2020-01-28T05:00:23Z
2020
 
Type Article
 
Identifier Planta, 251(2): 55
1432-2048
https://doi.org/10.1007/s00425-020-03346-w
https://pubmed.ncbi.nlm.nih.gov/31974682-novel-insights-into-expansion-and-functional-diversification-of-mir169-family-in-tomato/
http://223.31.159.10:8080/jspui/handle/123456789/1035
 
Language en_US
 
Format application/pdf
 
Publisher Springer Nature Publishing AG