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ath-miR164c influences plant responses to the combined stress of drought and bacterial infection by regulating proline metabolism

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Title ath-miR164c influences plant responses to the combined stress of drought and bacterial infection by regulating proline metabolism
 
Creator Gupta, Aarti
Patil, Mahesh
Qamar, Aarzoo
Senthil-Kumar, Muthappa
 
Subject Drought
Pseudomonas syringae
Physiological stress
microRNA
Proline
 
Description Accepted date: 22 January 2020
Plants under combined stresses exhibit a prominent shift in molecular responses compared with plants exposed
to the same stresses independently. Profiling responses to individual and combined stressors at the gene expression level have identified several genes with intersecting responses to these stressors. However, the upstream
regulators at the intersection of plant responses to individual and combined stresses are not known. Here, using
the transcriptome of Arabidopsis thaliana under individual and combined drought and Pseudomonas syringae
infection, we identified several genes whose expression overlaps between individual and combined stresses. To
study the key regulator of such an overlapping gene, we predicted that the expression of 1-Pyrroline-5-carboxylate
synthase 1 (AtP5CS1) is regulated by ath-miR164c at post-transcriptional level. Our results from the stem-loop
RT-PCR based expression analysis revealed significant downregulation of ath-miR164c in response to P. syringae
infection under both well-irrigated (pathogen only) and drought stress (combined stress) conditions.
Furthermore, an Arabidopsis loss-of-function mutant of the miRNA ath-miR164c exhibited resistance to pathogen
infection under combined stress, unlike the wild-type plants, implicating the role of ath-miR164c in regulating
plant immunity. AtP5CS1 gene expression and proline accumulation were enhanced in the ath-miR164c mutant
plants relative to the wild-type plants, demonstrating that ath-miR164c regulates AtP5CS1 of the proline biosynthesis pathway, which was also validated by 5’RLM-RACE results. This miRNA-mediated modulation of
AtP5CS1 gene expression under combined stress fills crucial gaps in identifying the key convergent players in the current understanding of plant stress responses.
Projects at MS-K lab are supported by DBT-Ramalingaswami re-entry fellowship grant (BT/RLF/re-entry/23/2012). AG (N-PDF/2015/000116) and MP (PDF/2016/000528) acknowledge the SERB National Post-Doctoral Fellowship. We also thank Mr. Sundar and Mr Rahim for extending labour at the laboratory and Mr. Ashok Kumar for technical help at central instrumentation facility. The authors are thankful to DBT-eLibrary Consortium (DeLCON) for providing access to e-resources. NIPGR Plant Growth Facility is also duly acknowledged. We thank Dr Muthamilarasan Mehanathan and Dr William Truman for critical comments on the manuscript. Also, we thank Dr Priya, Mr Vadivel, and Miss Aanchal for internally reviewing the manuscript.
 
Date 2020-01-29T08:59:59Z
2020-01-29T08:59:59Z
2020
 
Type Article
 
Identifier Environmental and Experimental Botany, 172: 103998
0098-8472
https://doi.org/10.1016/j.envexpbot.2020.103998
https://www.sciencedirect.com/science/article/pii/S0098847220300241
http://223.31.159.10:8080/jspui/handle/123456789/1036
 
Language en_US
 
Format application/pdf
 
Publisher Elsevier B.V.