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Calcium-dependent changes in physicochemical properties and the proteome dynamics influence dehydration responses in rice

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Title Calcium-dependent changes in physicochemical properties and the proteome dynamics influence dehydration responses in rice
 
Creator Rai, Yogita
Wardhan, Vijay
Gupta, Deepti Bhushan
Chakraborty, Niranjan
 
Subject Adaptive responses
Ca2+ -channels
Proteome profiling
Dehydration-responsive proteins
Ca2+ -signalling
MADS-box proteins
Oxidative damage
Phylogenetic relationship
 
Description Accepted date: 11 December 2019
The cytosolic Ca2+ ([Ca2+]cyt), in plants serves as secondary messenger during development and stress adaptive responses. While several of the components of Ca2+-signalling, especially involved in water-deficit stress or dehydration are known, the underlying mechanism of such regulations remain poorly understood. In this study, we investigated the Ca2+-mediated alleviation of dehydration stress in rice. The physicochemical indices of the rice seedlings pretreated with CaCl2, followed by dehydration treatment displayed better maintenance of relative water content (RWC) and cell membrane integrity, besides peroxide levels. CaCl2-pretreated seedling showed stimulation of antioxidants contributing to long-term survival under dehydration stress. Contrastingly, blocking of Ca2+-channels aggravated the dehydration-induced damage, suggesting a crucial role of Ca2+-signalling in stress adaptation. The cytosolic proteome profiling of CaCl2-pretreated seedlings revealed 100 distinct proteins that include 56 dehydration-responsive proteins (DRPs), presumably involved in adaptive responses. A critical screening of the proteome led to the identification of a MADS-box transcription factor family protein, designated OsMADS23. The predicted structure and nuclear localization indicated that OsMADS23 might bind to nucleic acids, suggesting its possible role in transcriptional regulation. The stimulation of stress-responsive expression of OsMADS23 by Ca2+ demonstrated its participation in Ca2+-dependent signalling. Altogether, these results indicate the Ca2+-dependent dehydration response in plants and substantiate the function of a MADS-box protein in the cross-talk of developmental and stress-responsive pathways.
This work was supported by grants from Department of
Biotechnology (DBT), India (BT/AGR/CG-PhaseII/01/2014) and
National Institute of Plant Genome Research, New Delhi to N.C. as well
as grants from DBT (BT//PR12745/27/170/2009) to D.B.G., pre-doctoral fellowship from DBT to Y.R., and pre-doctoral fellowship from
Council of Scientific and Industrial Research (CSIR), India to V.W. The
authors thank Mr. Jasbeer Singh for illustrations and graphical representation in the manuscript.
 
Date 2020-02-06T10:54:35Z
2020-02-06T10:54:35Z
2020
 
Type Article
 
Identifier Environmental and Experimental Botany, 172: 103965
0098-8472
http://223.31.159.10:8080/jspui/handle/123456789/1038
https://www.sciencedirect.com/science/article/pii/S0098847219315631
https://doi.org/10.1016/j.envexpbot.2019.103965
 
Language en_US
 
Format application/pdf
 
Publisher Elsevier B.V.