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Nested‐association mapping (NAM)‐based genetic dissection uncovers candidate genes for seed and pod weights in peanut ( Arachis hypogaea )

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Relation http://oar.icrisat.org/11371/
https://doi.org/10.1111/pbi.13311
doi:10.1111/pbi.13311
 
Title Nested‐association mapping (NAM)‐based genetic dissection uncovers candidate genes for seed and pod weights in peanut ( Arachis hypogaea )
 
Creator Gangurde, S S
Wang, H
Yaduru, S
Pandey, M K
Fountain, J C
Chu, Y
Isleib, T
Holbrook, C C
Xavier, A
Culbreath, A K
Ozias‐Akins, P
Varshney, R K
Guo, B
 
Subject Groundnut
Genetics and Genomics
Legume Crops
 
Description Multiparental genetic mapping populations such as nested-association mapping (NAM) havegreat potential for investigating quantitative traits and associated genomic regions leading torapid discovery of candidate genes and markers. To demonstrate the utility and power of thisapproach, two NAM populations, NAM_Tifrunner and NAM_Florida-07, were used for dissectinggenetic control of 100-pod weight (PW) and 100-seed weight (SW) in peanut. Two high-densitySNP-based genetic maps were constructed with 3341 loci and 2668 loci for NAM_Tifrunner andNAM_Florida-07, respectively. The quantitative trait locus (QTL) analysis identified 12 and 8major effect QTLs for PW and SW, respectively, in NAM_Tifrunner, and 13 and 11 major effectQTLs for PW and SW, respectively, in NAM_Florida-07. Most of the QTLs associated with PW andSW were mapped on the chromosomes A05, A06, B05 and B06. A genomewide associationstudy (GWAS) analysis identified 19 and 28 highly significant SNP–trait associations (STAs) inNAM_Tifrunner and 11 and 17 STAs in NAM_Florida-07 for PW and SW, respectively. Thesesignificant STAs were co-localized, suggesting that PW and SW are co-regulated by severalcandidate genes identified on chromosomes A05, A06, B05, and B06. This study demonstratesthe utility of NAM population for genetic dissection of complex traits and performing high-resolution trait mapping in peanut.
 
Publisher Wiley Open Access
 
Date 2019-12
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://oar.icrisat.org/11371/1/Gangurde_et_al-2019-Plant_Biotechnology_Journal.pdf
Gangurde, S S and Wang, H and Yaduru, S and Pandey, M K and Fountain, J C and Chu, Y and Isleib, T and Holbrook, C C and Xavier, A and Culbreath, A K and Ozias‐Akins, P and Varshney, R K and Guo, B (2019) Nested‐association mapping (NAM)‐based genetic dissection uncovers candidate genes for seed and pod weights in peanut ( Arachis hypogaea ). Plant Biotechnology Journal (TSI). pp. 1-15. ISSN 1467-7644