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Genome-wide identification and expression profile analysis of nuclear factor Y family genes in Sorghum bicolor L. (Moench)

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Relation http://oar.icrisat.org/11374/
https://doi.org/10.1371/journal.pone.0222203
doi:10.1371/journal.pone.0222203
 
Title Genome-wide identification and expression profile analysis of nuclear factor Y family genes in Sorghum bicolor L. (Moench)
 
Creator Mondal, T K
Maheshwari, P
Kummari, D
Palakolanu, S R
Nagasai Tejaswi, U.
Nagaraju, M.
Rajasheker, G.
Jawahar, G.
Jalaja, N.
Rathnagiri, P.
Kavi Kishor, P. B.
 
Subject Sorghum
Genetics and Genomics
 
Description Members of the plant Heme Activator Protein (HAP) or NUCLEAR FACTOR Y (NF-Y) are trimeric
transcription factor complexes composed of the NF-YA, NF-YB and NF-YC subfamilies.
They bind to the CCAAT box in the promoter regions of the target genes and regulate gene
expressions. Plant NF-Ys were reported to be involved in adaptation to several abiotic
stresses as well as in development. In silico analysis of Sorghum bicolor genome resulted in
the identification of a total of 42 NF-Y genes, among which 8 code for the SbNF-YA, 19 for
SbNF-YB and 15 for the SbNF-YC subunits. Analysis was also performed to characterize
gene structures, chromosomal distribution, duplication status, protein subcellular localizations,
conserved motifs, ancestral protein sequences, miRNAs and phylogenetic tree construction.
Phylogenetic relationships and ortholog predictions displayed that sorghum has additional
NF-YB genes with unknown functions in comparison with Arabidopsis. Analysis of promoters
revealed that they harbour many stress-related cis-elements like ABRE and HSE, but surprisingly,
DRE and MYB elements were not detected in any of the subfamilies. SbNF-YA1, 2, and
6 were found upregulated under 200 mM salt and 200 mM mannitol stresses. While NF-YA7
appeared associated with high temperature (40˚C) stress, NF-YA8 was triggered by both cold
(4˚C) and high temperature stresses. Among NF-YB genes, 7, 12, 15, and 16 were induced
under multiple stress conditions such as salt, mannitol, ABA, cold and high temperatures.
Likewise, NF-YC 6, 11, 12, 14, and 15 were enhanced significantly in a tissue specific manner
under multiple abiotic stress conditions. Majority of the mannitol (drought)-inducible genes
were also induced by salt, high temperature stresses and ABA. Few of the high temperature
stress-induced genes are also induced by cold stress (NF-YA2, 4, 6, 8, NF-YB2, 7, 10, 11, 12,
14, 16, 17, NF-YC4, 6, 12, and 13) thus suggesting a cross talk among them. This work paves
the way for investigating the roles of diverse sorghum NF-Y proteins during abiotic stress
responses and provides an insight into the evolution of diverse NF-Y members.
 
Publisher Public Library of Science
 
Date 2019-09
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://oar.icrisat.org/11374/1/file.pdf
Mondal, T K and Maheshwari, P and Kummari, D and Palakolanu, S R and Nagasai Tejaswi, U. and Nagaraju, M. and Rajasheker, G. and Jawahar, G. and Jalaja, N. and Rathnagiri, P. and Kavi Kishor, P. B. (2019) Genome-wide identification and expression profile analysis of nuclear factor Y family genes in Sorghum bicolor L. (Moench). PLOS ONE (TSI), 14 (9). pp. 1-27. ISSN 1932-6203