ICAR Research Data Repository for Knowledge Management
Comparative Evaluation Of Conventional And Real Time PCR For Detection Of Haemoparasites In Dogs
Online Publishing @ NISCAIR
View Archive Info| Field | Value | |
| Authentication Code |
dc |
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| Title Statement |
Comparative Evaluation Of Conventional And Real Time PCR For Detection Of Haemoparasites In Dogs |
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| Added Entry - Uncontrolled Name |
Wahlang, Lavinia Lakshmanan1, Bindu Thomas, Naicy |
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| Summary, etc. |
Ehrlichiosis and babesiosis are among the most pathogenic tick-borne diseases of dogs worldwide. The study reports the development of SYBR green based real time PCR protocols with novel primers targeting small subunit ribosomal RNA genes to detect natural infections of Ehrlichia canis, Babesia vogeli and B. gibsoni in dogs and its comparison with conventional PCR. Real time PCR could detect these prokaryotic and eukaryotic pathogens with 100% sensitivity. Statistical analysis revealed that real time PCR is more superior to conventional PCR assay to detect low level rickettsaemia (p<0.05). The high prevalence of these pathogens in the study population also warrants immediate attention to the adoption of efficient and sustainable control strategies. |
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| Publication, Distribution, Etc. |
Test Journal 2020-02-20 16:50:05 |
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| Electronic Location and Access |
http://op.niscair.res.in/index.php/TEST/article/view/32125 |
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| Data Source Entry |
Test Journal; ##issue.vol## 1, ##issue.no## 1 (2016): Test Journal new |
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| Language Note |
en |
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