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Transcript profiling reveals potential regulators for oxidative stress response of a necrotrophic chickpea pathogen Ascochyta rabiei

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Title Transcript profiling reveals potential regulators for oxidative stress response of a necrotrophic chickpea pathogen Ascochyta rabiei
 
Creator Maurya, Ranjeet
Singh, Yeshveer
Sinha, Manisha
Singh, Kunal
Mishra, Pallavi
Singh, Shreenivas Kumar
Verma, Sandhya
Prabha, Kanchan
Kumar, Kamal
Verma, Praveen K.
 
Subject Transcriptome
454 Pyrosequencing
Diferential expression profles
Oxidative stress
Stress response
 
Description Accepted date: 2 February 2020
Necrotrophic pathogens experience host-generated oxidative stress during pathogenesis. They overcome such hostile environment by intricate mechanisms which are largely understudied. In this article, reference-based transcriptome analysis of
a devastating Ascochyta Blight (AB) disease causing chickpea pathogen Ascochyta rabiei was explored to get insights into
survival mechanisms under oxidative stress. Here, expression profling of mock-treated and menadione-treated fungus was
carried out by RNA-Seq approach. A signifcant number of genes in response to oxidative stress were overrepresented, suggestive of a robust and coordinated defense system of A. rabiei. A total 73 diferentially expressed genes were fltered out
from both the transcriptomes, among them 64 were up-regulated and 9 were found down-regulated. The gene ontology and
KEGG mapping were conducted to comprehend the possible regulatory roles of diferentially expressed genes in metabolic
networks and biosynthetic pathways. Transcript profling, KEGG pathway and gene ontology-based enrichment analysis
revealed 12 (16.43%) stress responsive factors, 25 (34.24%) virulence associated genes, 10 (13.69%) putative efectors and
28 (38.35%) important interacting proteins associated with various metabolic pathways. In addition, genes with diferential
expression were further explored for underlying putative pathogenicity factors. We identifed fve genes ST47_g10291,
ST47_g9396, ST47_g10294, ST47_g4395, and ST47_g7191 that were common to stress and fungal pathogenicity. The
factors recognized in this work can be used to establish molecular tools to explain the regulatory gene networks engaged in
stress response of fungal pathogens and disease management.
This work is supported by external funds from Department of Biotechnology, Government of India (Project numbers: BT/PR7164/PBD/16/1016/2012 and BT/AGR/CG-Phase II/01/2014).
 
Date 2020-02-24T11:09:05Z
2020-02-24T11:09:05Z
2020
 
Type Article
 
Identifier 3 Biotech, 10: 117
2190-5738
https://doi.org/10.1007/s13205-020-2107-8
https://link.springer.com/article/10.1007/s13205-020-2107-8
http://223.31.159.10:8080/jspui/handle/123456789/1041
 
Language en_US
 
Format application/pdf
 
Publisher Springer Nature Publishing AG