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A novel compound β-sitosterol-3-O-β-D-glucoside isolated from <em>Azadirachta indica</em> effectively induces apoptosis in leukemic cells by targeting G0/G1 populations

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Title Statement A novel compound β-sitosterol-3-O-β-D-glucoside isolated from <em>Azadirachta indica</em> effectively induces apoptosis in leukemic cells by targeting G0/G1 populations
 
Added Entry - Uncontrolled Name Sarkar, Swadesh
Pal, Amrita
Chouni, Anirban
Paul, Santanu
 
Uncontrolled Index Term Anti-proliferative; Azadirachta indica; Cytotoxicity; Leukemia; β-sitosterol-3-O-β-D-glucoside
 
Summary, etc. <em>Azadirachta indica</em>, popularly known as ‘<em>Neem</em>’, is a very important plant in the Ayurveda system of medicine. It is known toprevent about 40 types of diseases in home-practice. A previous report showed that the methanolic extract of this plant can effectively control the proliferation of leukemia cells. Therefore, this research focuses on searching for a new molecule with potent anti-leukemic property. Ethanolic extract was prepared from the dried leaves. Four bio-molecules were isolated viz. rutin, isoquercetin, quercetrin and β-sitosterol-3-O-β-D-glucoside from the ethanolic extract by repeated column chromatography and HPLC. Quercetrin structures of the isolated molecules were confirmed by Mass, 1HNMR, 13C NMR spectra analysis. MTT assay revealed that β-sitosterol-3-O-β-D-glucosideeffectively reduced the proliferation of MOLT 4 leukemic cells in a dose and time dependent manner. DAPI staining with confocal microscopy indicated that β-sitosterol-3-O-β-D-glucoside efficiently induced nuclear DNA fragmentation in MOLT 4 cells. Finally, flow cytometry after PI staining showed that the compound has potential to check the cell cycle progression at sub G1 phase. In summary, we can conclude that β-sitosterol-3-O-β-D-glucoside has potential to be good therapeutic drug in leukemia treatment in future.
 
Publication, Distribution, Etc. Indian Journal of Biochemistry and Biophysics (IJBB)
2020-02-27 13:05:34
 
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http://op.niscair.res.in/index.php/IJBB/article/view/31770
 
Data Source Entry Indian Journal of Biochemistry and Biophysics (IJBB); ##issue.vol## 57, ##issue.no## 1 (2020): IJBB Vol.57(1) [February 2020]
 
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