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Title Functional Hydrolysates from yellow fin tuna red meat using RSM based optimization
 
Names Parvathy, U.
Binsi, P.K.
Joshy, C.G.
Jeyakumari, A.
Zynudheen, A.A.
Ninan, G.
Ravishankar, C.N.
Date Issued 2018 (iso8601)
Abstract The major hydrolytic variables for deriving functional hydrolysates from red meat of
yellow fin tuna (Thunnus albacares) were optimized using response surface methodology
(RSM). A central composite design (CCD) was employed to obtain the best possible
combination of enzyme-substrate ratio (X1: 0.25-1.5%) and hydrolysis duration (X2: 30-
240 min) to derive hydrolysates with strongest functional properties with emphasis on
protein recovery and sensory acceptability. The experimental data obtained were fitted to
second-order and third-order polynomial equation using multiple regression analysis. The
optimum hydrolytic conditions for superior functional properties were achieved at an
enzyme-substrate ratio (E/S) of 0.34 % for a hydrolysis duration of 30 minutes. The
protein recovery of the hydrolysates ranged between 32 - 50% which were linearly related
(R2 = 0.67) to the degree of hydrolysis. However a few properties of hydrolysate having
the same degree of hydrolysis varied significantly and hence, could not be entirely
explained based on the degree of hydrolysis
Genre Article
Identifier Int. J. Curr. Microbiol. Appl. Sci. 7(11):1462-1474