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| Field | Value |
| Title | Characterization and phylogenitic analaysis of ecotine biosynthesis genes from bacillus halodurans |
| Names |
Rajan, A.
Joseph, C.T. Thampuran, N. James, R. Viswanathan, C. Bansal, K.C. |
| Date Issued | 2008 (iso8601) |
| Abstract | Ectoine, a cyclic tetrahydropyrimidine (2- methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid), is a natural compound, which serves as a protective substance in many bacterial cells. In this study, the putative ectABC gene cluster from Bacillus halodurans was heterologously expressed in E. coli and the production of ectoine was con- Wrmed by HPLC analysis. The activity of the enzymes coded by the ectA, B and C genes were found to be higher in induced transgenic cells compared to the uninduced cells. Phylogenetic analysis revealed sequence identities ranging from 36–73% for ectA gene, 55–81% for ectB gene and 55–80% for ectC gene indicating that the enzymes are evolutionarily well conserved. |
| Genre | Article |
| Topic | B. halodurans |
| Identifier | Archives of Microbiology 2008: 190, 481-487 |