Record Details

DSpace at CIFT

View Archive Info
 

Metadata

 
Field Value
 
Title Molecular fingerprinting of salmonella enterica subsp. enterica typhimurium and salmonella enterica subsp. enterica derby isolated from tropical seafood in South India
 
Names Kumar, S.
Surendran, P.K.
Thampuran, N.
Date Issued 2008 (iso8601)
Abstract Salmonella enterica subsp. enterica Typhimurium
and Salmonella enterica subsp. enterica Derby strains
isolated from different seafood were genotyped by PCRribotyping
and ERIC-PCR assays. This study has ascertained
the genetic relatedness among serovars prevalent in
tropical seafood. PCR-ribotyping exhibited genetic variation
in both Salmonella serovars, and ribotype profile (II)
was most predominant, which was observed in 10/18 of
Salmonella enterica subsp. enterica Typhimurium and 7/17
Salmonella enterica subsp. enterica Derby isolates. Cluster
analysis of ERIC-PCR for Salmonella enterica subsp.
enterica Typhimurium strains exhibited nine different
banding patterns and four strains showed [95% genetic
homology within the cluster pairs. ERIC-PCR produced
more genetic variations in Salmonella enterica subsp.
enterica Typhimurium; nevertheless, both methods were
found to be comparable for Salmonella enterica subsp.
enterica Derby isolates. Discrimination index of PCRribotyping
for Salmonella enterica subsp. enterica
Typhimurium isolates was obtained at 0.674 and index
value 0.714 was observed for Salmonella enterica subsp.
enterica Derby strains. Molecular fingerprinting investigation
highlighted the hypothesis of diverse routes of
Salmonella contamination in seafood as multiple clones of
Salmonella enterica subsp. enterica Typhimurium and
Salmonella enterica subsp. enterica Derby were detected in
same or different seafood throughout the study period.
Genre Article
Topic Salmonella
Identifier Molecular Biotechnology 2008: 40(1), 95-100