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Title Studies on the microbial diversity of salted fishes under aerobic conditions
 
Names Rajan, L.A.
Joseph, T.C.
Thampuran, N.
Date Issued 2010 (iso8601)
Abstract AbA stottarla ocf t9 3 isolates of halophilic and moderately
halophilic bacteria were isolated from salted
fishes, Scoliodon sp. and Thrissina thryssa
obtained from retail fish outlets in Cochin,
Kerala, India. The viable count of the bacteria in
Scoliodon sp. and Thrissina thryssa ranged from
103-106 per g. The morphological, biochemical,
and physiological investigations were done to
characterize the isolates, which showed good
growth in medium containing 3-10% (w/v) NaCl
at 37°C. In Scoliodon sp. the maximum of 87.5%
isolates were Gram-positive cocci and in
Thrissina thryssa 95.5% of the isolates were
Gram-positive rods. The optimum conditions
such as temperature, pH, and salts (NaCl) were
determined. The utilization of organic compounds
such as fructose, lactose, glucose,
sucrose, arabinose, trehalose, maltose, salicin,
mannose, cellobiose, rhamnose, dulcitol, xylose,
raffinose, sorbitol, adonitol, inulin, galactose,
inositol, mannitol, hydrogen sulfide, nitrate,
citrate utilization, MR-VP, triple sugar iron, as
well as the hydrolysis of organic compounds
such as casein, gelatin, aesculin, starch, Tween
20, and Tween 80 were also investigated.
Moreover, an alkalophilic bacterial strain
Bacillus halodurans (B. halodurans) was isolated
from Thrissina thryssa and confirmed by 16S
rDNA analysis. These studies revealed that salted
fishes offer an optimal environment for the
viable, diverse, potentially and industrially
important bacterial community.
Genre Article
Topic Microbial diversity
Identifier Microbiology Research 2010: 2(4), 22-25