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Title Evaluation of azadirachta indica leaf fractions for in vitro antioxidant potential and protective effects against H2O2-induced oxidative damage to pBR322 DNA and red blood cells.
 
Names Manikandan, P.
Anandan, R.
Nagini, S.
Date Issued 2009 (iso8601)
Abstract We evaluated the protective effects of subfraction of the ethyl acetate fraction (EAF) and the methanolic faction (M/F) from the crude ethanolic extract (CEE) of Azadirachta indica A. Juss (neem) leaves against various free radicals and hydrogen peroxide (H2O2)-induced oxidative damage to red blood cells (RBCs) and pBR322 DNA. Neem leaf fraction reduced DPPH, ABTS+, superoxide (O-), hydroxyl (OH) , and nitric oxide radicals to nonradical forms in a concentration dependent manner. Treatment with the benzene insoluble fraction from EAF (EBIF), the chloroform insoluble fraction from EAF (ECIF), the chloroform insoluble fraction from MF (MCIF), and the ethyl acetate insoluble fraction from MF(MEIF) significantly mitagated H2O-induced oxidative damage to RBCs and pBR322 DNA. Although we found low in vitro free radical scavenging activity for the benzene insoluble fraction from EAF (EBSF), the chloroform soluble fraction from EAF (ECSF), the chloroform soluble fraction from MF (MCSF), and the ethyl acetate soluble fraction from MF (MESF), these fraction showed no effect on H2O-induced lipid peroxidation and pBR322 DNA damage. High-performance liquid chromatography (HPLC) and TLC-latroscan analysis revealed that the greater efficacy of EBIF, ECIF, MCIF, ad MEIF may be due to the presence of more polar compounds such as nimbolide and quercetin. Our studies suggest that the antioxidant and protective effects of active neem leaf fractions against H2O-induced lipid peroxidation and pBR322 DNA damage can be attributed to their ability to inhibit various free radicals
Genre Article
Identifier J. Agric. Food Chem. 57(15):6990-6996