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| Field | Value |
| Title | Polymerase chain reaction for the detection of white spot syndrome virus in post-larvae of penaeid shrimps |
| Names |
Joseph, T.C.
Surendran, P.K. |
| Date Issued | 2002 (iso8601) |
| Abstract | White spot syndrome is the most deadly viral disease of penaeid shrimps and has devastated shrimp aquaculture in many Asian countries. Affected shrimps are characterised by the presence of white spots on the carapace. A two step polymerase chain reaction(PCR) was used for the detection of latent state of white spot syndrome virus in post-larvae of penaeus monodon and P. indicus from hatcheries. The one step PCR gave an amplification product of 1447 bp and two step PCR a 941 bp product. The two step amplification with nested primers was found to be more sensitive than one step amplification. The sensitivity of two step amplification was 1000 times higher than the one step amplification. Of the post-larvae tested, 47% were found to be positive for white spot syndrome virus by two step PCR while only 22% were positive by one step PCR. |
| Genre | Article |
| Topic | Polymerase chain reaction |
| Identifier | Proceedings of the Symposium on Seafood Safety - Status and Strategies, 28-30 May 2002, Cochin, India ed. by Surendran, P.K. et. al, 422-426 |