Record Details

DSpace at CIFT

View Archive Info
 

Metadata

 
Field Value
 
Title Screening natural content of water soluble b vitamins in fish: enzymatic extraction, HILIC separation and tandem mass spectrometric determination
 
Names Chatterjee, N.S.
Ashok Kumar, K.
Ajeeshkumar, K.K.
Remya Kumari, K.R.
Vishnu, K.V.
Anandan, R.
Suseela Mathew
Ravishankar, C.N.
Date Issued 2017 (iso8601)
Abstract Despite the potential of LC with tandem MS (MS/MS)
in improving sensitivity and selectivity, analytical
methods are scarce for the determination of
protein-bound and phosphorylated forms of B
vitamins in food. This prompted us to develop a
method for LC-MS/MS determination of naturally
occurring nicotinamide, nicotinic acid, thiamine,
pyridoxine, riboflavin, pantothenic acid, biotin,
folic acid, and cyanocobalamin in fish. Baseline
separation of the vitamins was achieved in
a hydrophilic interaction LC condition. An
ultrasonication-assisted enzymatic extraction
protocol for sample preparation was optimized
and validated. The time required for extraction was
significantly reduced (to 4 h), while maintaining good
extraction efficiency. Acetonitrile content (80%, v/v)
in the prepared sample was found to be optimum for
excellent peak shape and sensitivity. The dynamic
linear range of the vitamins ranged from 2.5 to
500 ng/g, and the regression coefficient values were
greater than 0.99. LOQ values ranged from 0.4 to
50 ng/g for the different vitamins. The spike recovery
values at 50 and 100 ng/g ranged from
87.5 to 97.5%. The intra- and interday precision
values were satisfactory. Accuracy of the developed
method was determined by analysis of a Certified
Reference Material. The method could also be
used for unambiguous determination of the natural
content of the target vitamins in fish.
Genre Article
Identifier J. AOAC Int. 100(3): 579-585