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Title Optimization of process parameters for the production of collagen peptides from fish skin (epinephelus malabaricus) using response surface methodology and its characterization.
 
Names Hema, G.S.
Joshy, C.G.
Shyni, K.
Chatterjee, N.S.
George Ninan
Suseela Mathew
Date Issued 2017 (iso8601)
Abstract The study optimized the hydrolysis conditions
for the production of fish collagen peptides from skin of
Malabar grouper (Epinephelus malabaricus) using
response surface methodology. The hydrolysis was done
with enzymes pepsin, papain and protease from bovine
pancreas. Effects of process parameters viz: pH, temperature,
enzyme substrate ratio and hydrolysis time of the
three different enzymes on degree of hydrolysis were
investigated. The optimum response of degree of hydrolysis
was estimated to be 10, 20 and 28% respectively for
pepsin, papain and protease. The functional properties of
the product developed were analysed which showed
changes in the properties from proteins to peptides. SDSPAGE
combined with MALDI TOF method was successfully
applied to determine the molecular weight distribution
of the hydrolysate. The electrophoretic pattern
indicated that the molecular weights of peptides formed
due to hydrolysis were nearly 2 kDa. MALDI TOF spectral
analysis showed the developed hydrolysate contains peptides
having molecular weight in the range below 2 kDa.
Genre Article
Topic Fish collagen peptide
Identifier J. Food Sci. Technol. 54(2) : 488-496