ICAR Research Data Repository for Knowledge Management
DSpace at CIFT
View Archive InfoMetadata
| Field | Value |
| Title | Genetic heterogeneity of non-O1 and non-O139 Vibrio cholerae isolates from shrimp aquaculture system: a comparison of RS-, REP- and ERIC-PCR fingerprinting approaches |
| Names |
Rao, B.M.
Surendran, P.K. |
| Date Issued | 2010 (iso8601) |
| Abstract | The genetic diversity of Vibrio cholerae isolated from black tiger shrimp (Penaeus monodon) aquaculture farms was determined using three PCR typing methods based on enterobacterial repetitive intergenic consensus (ERIC) sequences, ribosomal gene spacer (RS) sequence and repetitive extragenic palindromic (REP) sequences.Non-O1 and non-O139 V. cholerae isolates were obtained from shrimp pond water, pond sediment, shrimp head and shrimp muscle. RS-PCR yielded fewer bands than REP-PCR and ERIC-PCR. Higher similarity was observed in RS-PCR (75–100%) than in REP-PCR (60–95%) and ERIC-PCR (40–95%).The choleratoxigenic V. cholerae (non-O1 and non-O139) showed greater genetic similarity with ctx-negative V. cholerae than among ctx-positive V. cholerae.The greater similarity of ctx-positive V. cholerae with ctx-negative V. cholerae isolates indicates that the ctx-positive strains (non-O1 and non-O139) might have originated from autochthonous V. cholerae in the aquatic niche. |
| Genre | Article |
| Topic | Black tiger shrimp |
| Identifier | Letters in Applied Microbiology 2010:51(1), 65-74 |