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Integrated seed proteome and phosphoproteome analyses reveal interplay of nutrient dynamics, carbon-nitrogen partitioning and oxidative signaling in chickpea

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Title Integrated seed proteome and phosphoproteome analyses reveal interplay of nutrient dynamics, carbon-nitrogen partitioning and oxidative signaling in chickpea
 
Creator Sinha, Arunima
Haider, Toshiba
Narula, Kanika
Ghosh, Sudip
Chakraborty, Niranjan
Chakraborty, Subhra
 
Subject Seed
Nutrient
proteome & phosphoproteome
2DE
Mass spectrometry
Chickpea
 
Description Accepted date: 6th March 2020
Nutrient dynamics in storage organs is a complex developmental process that requires coordinated interactions of environmental, biochemical, and genetic factors. Although sink organ developmental events have been identified, our understanding of translational and post‐translational regulation of reserve synthesis, accumulation and utilization in legume crops is limited. To understand nutrient dynamics during embryonic and cotyledonary photoheterotrophic transition to mature and germinating autotrophic seeds, an integrated proteomics and phosphoproteomics study in six sequential seed developmental stages in chickpea was performed. MS/MS analyses identified 109 unique nutrient‐associated proteins (NAPs) involved in metabolism, storage and biogenesis, and protein turnover. Differences and similarities in 60 nutrient‐associated phosphoproteins (NAPPs) containing 93 phosphosites were compared with NAPs. Data revealed accumulation of carbon‐nitrogen metabolic and photosynthetic proteoforms during seed filling. Furthermore, enrichment of storage proteoforms and protease inhibitors was associated with cell expansion and seed maturation. Finally, combined proteoforms network analysis identified three significant modules, centered around malate dehydrogenase, HSP70, triose phosphate isomerase and vicilin. Novel clues suggest that ubiquitin‐proteasome pathway regulates nutrient reallocation. Second, increased abundance of NAPs/NAPPs related to oxidative and serine/threonine signalling indicate direct interface between redox sensing and signaling during seed development. Taken together, nutrient signals act as metabolic and differentiation determinant governing storage organ reprogramming.
This work was supported by grants from Department of Biotechnology,
Govt. of India (No. BT/AGR/CG-Phase II/01/2014, BT/PR 12919/AGR/02/676/2009 and
BT/HRD/35/01/05/2013) and National Institute of Plant Genome Research, New Delhi, India
to S.C. A.S. is the recipient of pre-doctoral fellowship from the Council of Scientific and
Industrial research (CSIR), Govt. of India. T. H. is the recipient of post-doctoral fellowship
from the Council of Scientific and Industrial research (CSIR), Govt. of India. Financial
support from the DBT-RA program in Biotechnology and Life Sciences to K. N. is gratefully
acknowledged. S.G. is the recipient of Science and Engineering Research Board (SERB),
DST, Govt. of India. Authors also thank Jasbeer Singh for illustrations and graphical
representations in the manuscript.
 
Date 2020-03-11T05:36:30Z
2020-03-11T05:36:30Z
2020
 
Type Article
 
Identifier Proteomics, 20: 1900267
1615-9861
http://223.31.159.10:8080/jspui/handle/123456789/1045
 
Language en_US
 
Format application/pdf
 
Publisher John Wiley & Sons