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Title Prevalence of methicillin resistant staphylococcus aureus in selected seafood markets and aquaculture farms in Kerala, south-west coast of India
 
Names Murugadas, V.
Joseph, T.C.
Reshmi, K.
Lalitha, K.V.
Date Issued 2016 (iso8601)
Abstract A study was carried out to understand the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in selected
seafood commodities, market environment as well as in aquaculture farms in Kerala, along the south-west coast of India.
Two hundred and thirty three samples comprising finfish, crustaceans, molluscs and fishery environmental samples from
markets as well as aquaculture farms were collected and screened for the presence of MRSA. The S. aureus isolates obtained
from the samples were checked for resistance to methicillin, oxacillin and cefoxitin by antimicrobial sensitivity test and
further confirmed by oxacillin agar screening method. A multiplex PCR was employed targeting mecA, nuc and 16s rDNA
genes to differentiate S. aureus and MRSA from other staphylococci. It was found that 13.4% of the samples harboured
MRSA while the overall prevalence of S. aureus was found to be 36.5%. This study has clearly indicated high prevalence
of methicillin resistant S. aureus strains in the seafood as well as in environmental samples from seafood markets and
aquaculture farms in Kerala. The prevalence of methicillin resistant strains of staphylococci in fish indicates high risk
associated with fish handlers and fish farmers.
Genre Article
Topic mecA gene
Identifier Indian J. Fish 63(4): 150-153