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Identification of polymorphic SSR markers in elite genotypes of pearl millet and diversity analysis

OAR@ICRISAT

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Relation http://oar.icrisat.org/11396/
https://doi.org/10.1016/j.egg.2019.100051
doi:10.1016/j.egg.2019.100051
 
Title Identification of polymorphic SSR markers in elite genotypes of pearl millet and diversity analysis
 
Creator Kumar, S
Hash, C T
Singh, G
Basava, R K
Srivastava, R K
 
Subject Pearl Millet
Genetics and Genomics
Food and Nutrition
 
Description Pearl millet is a climate-resilient crop of marginal agro-ecologies and semi-arid tropics of Asia and Africa. With
substantial nutritional qualities crop requires low inputs and is capable of giving economic returns. Development
of high-yielding hybrids is an important breeding objective for pearl millet worldwide. The knowledge of genetic
diversity is a prerequisite for developing superior hybrids. In the current study, attempts were made to evaluate
the diversity of 17 important Indian pearl millet inbred genotypes and one popular hybrid 9444 using fluorescent
labeled SSR markers. A total of 342 polymorphic alleles with an average of 4.62 alleles per primer were produced
from 74 SSR markers. Polymorphic information content (PIC) ranged from 0.10 to 0.89 with an average of 0.55.
A very low level of heterozygosity was detected in genotypes. The average genetic dissimilarity detected between
pairs of inbred lines was 0.66. Genetic dissimilarity estimates calculated among the inbred lines varied from
0.108 (AIMP-03 and AIMP-08) to a maximum of 0.851(AIMP-03/AIMP-08 and 81B). The results indicated that
sufficient genetic variability is available in studied genotypes which can be exploited through heterosis breeding
to develop hybrids. The study also presents a suit of SSR markers that could be effectively used for genetic diversity
analysis in pearl millet.
 
Date 2020-02
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://oar.icrisat.org/11396/1/main.pdf
Kumar, S and Hash, C T and Singh, G and Basava, R K and Srivastava, R K (2020) Identification of polymorphic SSR markers in elite genotypes of pearl millet and diversity analysis. Ecological Genetics and Genomics, 14. ISSN 2405-9854